概述

  • 产品名称
  • 描述
    山羊多克隆抗体to FSTL1
  • 宿主
    Goat
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide: TAEKTKRVSTKEI, corresponding to amino acids 296-308 of Human FSTL1.

  • 阳性对照
    • Human Placenta and Ovary lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/16000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human FSTL1 peptide (ab23213). The observed molecular weight corresponds to earlier findings in literature (Tanaka et al, Int Immunol. 1998 Sep;10(9):1305-14.

靶标

图片

  • Anti-FSTL1 antibody (ab11805) at 0.5 µg/ml + Human Placenta lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size: 35 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

实验方案

文献

This product has been referenced in:
  • Sundaram GM  et al. EGF hijacks miR-198/FSTL1 wound-healing switch and steers a two-pronged pathway toward metastasis. J Exp Med 214:2889-2900 (2017). Read more (PubMed: 28827448) »
  • Mouillet JF  et al. The expression and post-transcriptional regulation of FSTL1 transcripts in placental trophoblasts. Placenta 36:1231-8 (2015). Read more (PubMed: 26386648) »

See all 6 Publications for this product

客户评价及客户问答

Thank you for your reply.

Unfortunately it won't be possible to send the replacement to your collaborator.

What about ab107431?This rabbit polyclonal antibody is directed against the C-terminal part of Human FSTL1 and the immunogen...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (mesenchymal stem cells)
Loading amount
8 µg
Specification
mesenchymal stem cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

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Verified customer

提交于 May 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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