Anti-FSTL1/FRP抗体(ab111969)
Key features and details
- Rabbit polyclonal to FSTL1/FRP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FSTL1/FRP抗体
参阅全部 FSTL1/FRP 一抗 -
描述
兔多克隆抗体to FSTL1/FRP -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in Human Plasma Total Protein lysate as well as the following whole cell lysates: A431; MD MBA 231; HeLa; NIH3T3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111969于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 35 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 35 kDa). |
靶标
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功能
May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. -
组织特异性
Overexpressed in synovial tissues from rheumatoid arthritis. -
序列相似性
Contains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 534482 Cow
- Entrez Gene: 11167 Human
- Entrez Gene: 14314 Mouse
- Entrez Gene: 100172767 Orangutan
- Entrez Gene: 79210 Rat
- Omim: 605547 Human
- SwissProt: Q58D84 Cow
- SwissProt: Q12841 Human
see all -
别名
- Anti FSTL1 antibody
- FLJ50214 antibody
- FLJ52277 antibody
see all
图片
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All lanes : Anti-FSTL1/FRP antibody (ab111969) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 11 kDa (possible non-specific binding), 48 kDa (possible cleavage fragment), 95 kDa (possible non-specific binding)
Exposure time: 2 minutesFSTL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The band observed at 48 kDa could potentially be a cleaved form of FSTL1 due to the presence of a 20amino acid signal peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111969 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab111969 被引用在 1 文献中.
- Wang WL et al. The lncRNA PVT1/miR-590-5p/FSTL1 axis modulates the proliferation and migration of airway smooth muscle cells in asthma. Autoimmunity 54:138-147 (2021). PubMed: 33825599