概述

  • 产品名称Anti-FSH Receptor抗体
    参阅全部 FSH Receptor 一抗
  • 描述
    兔多克隆抗体to FSH Receptor
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Guinea pig, Pig, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human FSH Receptor.

  • 阳性对照
    • This antibody gave a positive signal in Rat Ovary tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab103874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

靶标

  • 功能Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • 组织特异性Sertoli cells and ovarian granulosa cells.
  • 疾病相关Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
    Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 翻译后修饰N-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Follicle stimulating hormone receptor antibody
    • Follicle stimulating hormone receptor isoform 1 antibody
    • Follicle-stimulating hormone receptor antibody
    • Follitropin receptor antibody
    • FSH receptor antibody
    • FSH-R antibody
    • Fshr antibody
    • FSHR_HUMAN antibody
    • FSHRO antibody
    • LGR1 antibody
    • MGC141667 antibody
    • MGC141668 antibody
    • ODG1 antibody
    • ovarian dysgenesis 1 antibody
    see all

Anti-FSH Receptor antibody 图像

  • Anti-FSH Receptor antibody (ab103874) at 1 µg/ml + Ovary (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 14 kDa,230 kDa,28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab103874 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103874, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.

Anti-FSH Receptor antibody (ab103874)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for your reply.

Do you have access to rat or human tissue sections to run a positive control? I do agree that the localization should be in the granulosa cells, but the trouble with this is that the antibody is not validated for ham...

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Thank you for your inquiry.


We haven't tested this antibody on golden hamster ovary tissue sections, so we are unsure how or if it will cross-react.


I hope this information helps. Please contact us with any other questions.

DISCOUNT CODE: *** Expiration date: April 9, 2012 I am very pleased to hear you would like to accept our offer and test ab113421 in hamster samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, pleas...

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