Anti-FRYL抗体(ab95065)

概述

  • 产品名称Anti-FRYL抗体
    参阅全部 FRYL 一抗
  • 描述
    兔多克隆抗体to FRYL
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).

  • 阳性对照
    • HeLa whole cell lysate, 293T cell lysate, Mouse NIH3T3 cell lysate,

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab95065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 340 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.
  • 组织特异性Widely expressed with higher expression in colon, placenta, brain and cells of lymphoid origin.
  • 疾病相关A chromosomal aberration involving FRYL is found in treatment-related acute lymphoblastic leukemia (ALL). Translocation t(4;11)(p12;q23) that forms a KMT2A/MLL1-FRYL fusion protein.
  • 序列相似性Belongs to the furry protein family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2010313D22Rik antibody
    • 2310004H21Rik antibody
    • 2510002A14Rik antibody
    • 9030227G01Rik antibody
    • AF4P12 antibody
    • AI930088 antibody
    • ALL fused gene from chromosome 4p12 antibody
    • ALL1-fused gene from chromosome 4p12 protein antibody
    • DKFZp686E205 antibody
    • FLJ161772 antibody
    • FRY like antibody
    • FRYL antibody
    • FRYL_HUMAN antibody
    • Furry homolog like (Drosophila) antibody
    • Furry homolog like antibody
    • Furry like antibody
    • KIAA0826 antibody
    • mKIAA0826 antibody
    • Protein furry homolog-like antibody
    see all

Anti-FRYL antibody 图像

  • All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 340 kDa


    Exposure time : 10 seconds
  • Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
    Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
    Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

    Subsequent Western blot detection used ab95065 at 1 µg/ml.

    Developed using the ECL technique with an exposure time of 1 seconds.

Anti-FRYL antibody (ab95065)参考文献

ab95065 has not yet been referenced specifically in any publications.

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Our datasheet (http://www.abcam.com/fryl-antibody-ab95065.html) shows data similar to what your are seeing: many bands at lower molecular weights but also a single band at the expected size. These sampl...

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