概述

  • 产品名称Anti-FRS2抗体
    参阅全部 FRS2 一抗
  • 描述
    兔多克隆抗体to FRS2
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 487-505 of Human FRS2alpha.

  • 阳性对照
    • Rat PC-12 and human HepG2 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab10425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. Use 10µg to 20ug using 3 mg RIPA lysate of mouse NIH/3T3 cells.
WB 1/1000. Detects a band of approximately 92, 95 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
  • 组织特异性Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
  • 序列相似性Contains 1 IRS-type PTB domain.
  • 翻译后修饰Phosphorylated on tyrosine residues upon stimulation by NGF.
    Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.
  • 细胞定位Endomembrane system. Cytoplasmic, membrane-bound.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FGFR signaling adaptor SNT antibody
    • FGFR signalling adaptor antibody
    • FGFR substrate 2 antibody
    • FGFR-signaling adaptor SNT antibody
    • Fibroblast growth factor receptor substrate 2 antibody
    • FRS 2 antibody
    • FRS2 antibody
    • FRS2_HUMAN antibody
    • FRS2A antibody
    • FRS2alpha antibody
    • SNT 1 antibody
    • SNT antibody
    • SNT-1 antibody
    • SNT1 antibody
    • Suc 1 Associated Neurotrophic Factor Target antibody
    • Suc1 associated neurotrophic factor target 1 antibody
    • Suc1-associated neurotrophic factor target 1 antibody
    see all

Anti-FRS2 antibody 图像

  • Immunohistochemical analysis of murine aorta tissue, staining FRS2 with ab10425.

    Tissue was fixed with paraformaldehyde and blocked with 10% BSA and horse serum; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in 10% BSA and horse serum) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

Anti-FRS2 antibody (ab10425)参考文献

This product has been referenced in:
  • Li H  et al. Frs2a and Shp2 signal independently of Gab to mediate FGF signaling in lens development. J Cell Sci 127:571-82 (2014). Read more (PubMed: 24284065) »
  • Tchaicha JH  et al. Kinase domain activation of FGFR2 yields high-grade lung adenocarcinoma sensitive to a Pan-FGFR inhibitor in a mouse model of NSCLC. Cancer Res 74:4676-84 (2014). Read more (PubMed: 25035393) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (aorta cross section)
Specification aorta cross section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
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Verified customer

提交于 Jan 03 2013

Thank you for your patience.

FRS2 is known to show a doublet around 92-95 kDa, as is reported in this paper: http://www.jbc.org/content/274/16/11321.full

The monomer form of FRS2 has a predicted molecular weight of 57 kDa, but the p...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry to learn that you are having difficulty getting the correct signal with ab10425. As we d...

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Thank you for providing your protocol details and an image of your blot. The originator of this antibody was able to provide the following information regarding the Western blot conditions that were originally used. Control: Human HepG2 cell lysate...

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Thank you for your enquiry. We don't produce ab10425 in-house, it is sourced from outside Abcam. I can't reveal the name of the originator, but I can tell you that we do not obtain the antibody from Santa Cruz. If you have any more questions, please do...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"