概述

  • 产品名称Anti-FRMD6抗体
    参阅全部 FRMD6 一抗
  • 描述
    兔多克隆抗体to FRMD6
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 446-580 of Human FRMD6 (Q96NE9).

  • 阳性对照
    • Human duodenum tissue; RT-4, EFO-21, A-431, Liver, Tonsil cell lysates; and Human cell line U-2 OS.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

Anti-FRMD6 antibody 图像

  • Immunohistochemical staining of human duodenum with anti-FRMD6 antibody (ab121133) at 1:350 dilution. It shows strong cytoplasmic positivity in glandular cells
  • All lanes : Anti-FRMD6 antibody (ab121133) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A-431 cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • Immunofluorescent staining of human cell line U-2 OS (fixed/permealized with PFA/Trion X-100) with anti-FRMD6 antibody (ab121133) at 1µg/ml in green. It shows positivity in mitochondria.

Anti-FRMD6 antibody (ab121133)参考文献

ab121133 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121133.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"