Anti-FRMD5抗体(ab118737)

概述

  • 产品名称Anti-FRMD5抗体
  • 描述
    兔多克隆抗体to FRMD5
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 164-213 (FPKHSEKLEK KIAEIHKTEL SGQTPATSEL NFLRKAQTLE TYGVDPHPCK) of Rat FRMD5 (XP_230513).

  • 阳性对照
    • Rat Muscle lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-FRMD5 antibody 图像

  • Anti-FRMD5 antibody (ab118737) at 1 µg/ml + Rat Muscle lysate at 10 µg

    Predicted band size : 65 kDa

Anti-FRMD5 antibody (ab118737)参考文献

ab118737 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview for ab118737. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species (mouse), we would like to follow up on thi...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (cerebellum)
Loading amount 100 µg
Specification cerebellum
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Fuzi Jin

Verified customer

提交于 Nov 28 2012

Thank you for your phone call.
I am sorry to hear that the antibody did not work in mouse samples. Unfortunately, we have not yet tested mouse samples and thus do not know if and how well the antibody would work.

I am sending you the disco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"