antigen sequence, corresponding to amino acids 899-1009 of Human FRMD4B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling. May function as a scaffolding protein.
Contains 1 FERM domain.
Cytoplasm > cytoskeleton.
Information by UniProt
- 6030440G05Rik antibody
- FERM domain-containing protein 4B antibody
- FRM4B_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-FRMD4B antibody (ab121310)
Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus and centrosome. Recommended concentration of ab121310 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FRMD4B antibody (ab121310)
ab121310 at 1/200 dilution staining FRMD4B in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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