概述

  • 产品名称
    Anti-FRMD4A抗体
  • 描述
    兔多克隆抗体to FRMD4A
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: EGAHDKGAGR AAVSDELRQW YQRSTASHKE HSRLSHTSST SSDSGSQYST SSQSTFVAHS RVTRMPQMCK ATSA, corresponding to amino acids 912-985 of Human FRMD4A.

  • 阳性对照
    • Human testis tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and golgi apparatus. Recommended concentration of ab122475 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122475 at 1/50 dilution staining FRMD4A in paraffin-embedded Human testis tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Fan Y  et al. Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer. Exp Ther Med 11:421-426 (2016). Read more (PubMed: 26893625) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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