Anti-Frizzled 9抗体(ab102956)

概述

  • 产品名称Anti-Frizzled 9抗体
    参阅全部 Frizzled 9 一抗
  • 描述
    兔多克隆抗体to Frizzled 9
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Cow, Dog, Human, Pig, Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Mouse Frizzled 9 aa 550 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9R216

  • 阳性对照
    • This antibody gave a positive signal in the following Mouse tissue lysates: E12 Embryo Brain and Spinal cord; Eye; Testis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).

靶标

  • 功能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 组织特异性Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney.
  • 序列相似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 结构域Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 349 antibody
    • CD 349 antigen antibody
    • CD349 antibody
    • Frizzled Drosophila homolog of 3 antibody
    • Frizzled homolog 9 (Drosophila) antibody
    • Frizzled homolog 9 antibody
    • Frizzled-9 antibody
    • Fz 9 antibody
    • Fz-9 antibody
    • FZD 3 antibody
    • Fzd 9 antibody
    • FZD3 antibody
    • FZD3-PEN antibody
    • FZD9 antibody
    • FZD9_HUMAN antibody
    • FzE6 antibody
    • hFz9 antibody
    • mfz9 antibody
    • Xfz9 antibody
    see all

Anti-Frizzled 9 antibody 图像

  • All lanes : Anti-Frizzled 9 antibody (ab102956) at 1 µg/ml

    Lane 1 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Eye (Mouse) Whole Cell Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa (possible non-specific binding),50 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    Frizzled 9 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab102956 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-Frizzled 9 antibody (ab102956)参考文献

ab102956 has not yet been referenced specifically in any publications.

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