The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
Use a concentration of 3 µg/ml.
功能Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.
组织特异性Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.
序列相似性Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.
结构域The PDZ-binding motif mediates interaction with GOPC. Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway. The FZ domain is involved in binding with Wnt ligands.
翻译后修饰Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
细胞定位Membrane. Golgi apparatus. Cell membrane. Colocalizes with GOPC at the Golgi apparatus.
ICC/IF image of ab40012 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40012 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - Frizzled 8 antibody (ab40012)
Anti-Frizzled 8 antibody (ab40012) at 1 µg/ml + Human Pancreas lysate in RIPA buffer at 35 µg
ab40012 at 3µg/ml staining Frizzled 8 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Anti-Frizzled 8 antibody (ab40012)参考文献
This product has been referenced in:
Gammons MV et al. Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. J Cell Sci129:3892-3902 (2016).
Read more (PubMed: 27744318) »