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Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human Frizzled 7.
(Peptide available as ab64635.)
Our Abpromise guarantee covers the use of ab64636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 23251385|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 64 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab64636 (1/250) staining Frizzled 7 in paraffin-embedded Human kidney (papillary cancer) tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.
Immunohistochemical image of ab64636 (dilution 1/2000) staining in rat spinal cord PFA fixed frozen tissue section, performed on an Olympus BX61 Motorized Research Microscope. The scale bar represents 50 µm.