概述

  • 产品名称Anti-Frizzled 5抗体
    参阅全部 Frizzled 5 一抗
  • 描述
    兔多克隆抗体to Frizzled 5
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human Frizzled 5.

  • 阳性对照
    • LOVO cell extracts. A549 cells.

性能

应用

Our Abpromise guarantee covers the use of ab75234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
  • 序列相似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 结构域The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C2orf31 antibody
    • DKFZP434E2135 antibody
    • Frizzled 5 seven transmembrane spanning receptor antibody
    • Frizzled class receptor 5 antibody
    • Frizzled family receptor 5 antibody
    • Frizzled homolog 5 antibody
    • Frizzled-5 antibody
    • Frizzled5 antibody
    • Fz 5 antibody
    • Fz-5 antibody
    • Fz5 antibody
    • FZD 5 antibody
    • fzd5 antibody
    • FZD5_HUMAN antibody
    • FzE 5 antibody
    • FzE5 antibody
    • HFZ 5 antibody
    • hFz5 antibody
    • MGC129692 antibody
    • Seven transmembrane receptor frizzled 5 antibody
    • Uncharacterized protein C2orf31 antibody
    • Wnt receptor antibody
    see all

Anti-Frizzled 5 antibody 图像

  • ab75234 at 1/500 dilution staining Frizzled 5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Frizzled 5 antibody (ab75234) at 1/500 dilution

    Lane 1 : LOVO cell extracts
    Lane 2 : LOVO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • ab75234 (4µg/ml) staining Frizzled 5 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the Islets of Langerhans.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Frizzled 5 antibody (ab75234)参考文献

This product has been referenced in:
  • Shen YL  et al. Bone marrow mesenchymal stem cell-derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non-canonical Wnt signaling pathway. Oncol Lett 8:85-90 (2014). WB . Read more (PubMed: 24959224) »
  • Arderiu G  et al. Monocyte-secreted Wnt5a interacts with FZD5 in microvascular endothelial cells and induces angiogenesis through tissue factor signaling. J Mol Cell Biol N/A:N/A (2014). Read more (PubMed: 25240054) »

See all 6 Publications for this product

Product Wall

Thank you for your patience in this matter.
I have been in contact with the lab about ab75234 and we are having the peptide used to generate the antibody added to the catalogue. That way I can send it to you to use as a blocking peptide and determ...

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Thank you for your reply..
Firstly, I would like to assure you that the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on mouse samples . If we cannot resolve the issue you are having with the anti...

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Thank you for contacting Abcam.
I have attached your image, with an arrow pointing to the band that I think is Frizzled 5. However is the banding pattern that you were expecting to see between your samples?
I would be more than happy to help ...

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Thank you for contacting us last week. Unfortunately we do not stock the immunogen peptide for ab75234 (anti-frizzled 5 antibody). Apologies for any inconvenience.

Thank you for your reply. I am sorry to confirm that ab58177 been tested on recombinant protein only, and not on endogenous samples. Therefore, as requested,  I have asked our Finance department to issue a credit note for you. Credit note ID: 18...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Thank you for providi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"