Anti-Frizzled 5抗体(ab58177)

概述

  • 产品名称Anti-Frizzled 5抗体
    参阅全部 Frizzled 5 一抗
  • 描述
    小鼠单克隆抗体to Frizzled 5
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: PLVEIQCSPD LRFFLCSMYT PICLPDYHKP LPPCRSVCER AKAGCSPLMR QYGFAWPERM SCDRLPVLGR DAEVLCMDYN RSEATTAPPR , corresponding to amino acids 72-162 of Human Frizzled 5

性能

应用

Our Abpromise guarantee covers the use of ab58177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

靶标

  • 功能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
  • 序列相似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 结构域The PDZ-binding motif mediates interaction with GOPC.
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Cell membrane. Golgi apparatus membrane. Localized at the plasma membrane and also found at the Golgi.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C2orf31 antibody
    • DKFZP434E2135 antibody
    • Frizzled 5 seven transmembrane spanning receptor antibody
    • Frizzled class receptor 5 antibody
    • Frizzled family receptor 5 antibody
    • Frizzled homolog 5 antibody
    • Frizzled-5 antibody
    • Frizzled5 antibody
    • Fz 5 antibody
    • Fz-5 antibody
    • Fz5 antibody
    • FZD 5 antibody
    • fzd5 antibody
    • FZD5_HUMAN antibody
    • FzE 5 antibody
    • FzE5 antibody
    • HFZ 5 antibody
    • hFz5 antibody
    • MGC129692 antibody
    • Seven transmembrane receptor frizzled 5 antibody
    • Uncharacterized protein C2orf31 antibody
    • Wnt receptor antibody
    see all

Anti-Frizzled 5 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab58177 Frizzled 5 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

Anti-Frizzled 5 antibody (ab58177)参考文献

ab58177 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. I am sorry to confirm that ab58177 been tested on recombinant protein only, and not on endogenous samples. Therefore, as requested,  I have asked our Finance department to issue a credit note for you. Credit note ID: 18...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Diverse cell types)
Loading amount 20 µg
Specification Diverse cell types
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Rauner

Verified customer

提交于 Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"