概述

  • 产品名称Anti-Frizzled 4抗体
    参阅全部 Frizzled 4 一抗
  • 描述
    山羊多克隆抗体to Frizzled 4
  • 特异性Tested in human and expected to react in rat given sequence similarity
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken
  • 免疫原

    Synthetic peptide:

    DEEERRCDPIRIS

    , corresponding to amino acids 39-51 of Human Frizzled 4 (NP_036325.2).

  • 阳性对照
    • Approx 60kDA band observed in Human Colon and Small Intestine

性能

应用

Our Abpromise guarantee covers the use of ab77724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/16000.

靶标

  • 功能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 组织特异性Almost ubiquitous. Largely expressed in adult heart, skeletal muscle, ovary, and fetal kidney. Moderate amounts in adult liver, kidney, pancreas, spleen, and fetal lung, and small amounts in placenta, adult lung, prostate, testis, colon, fetal brain and liver.
  • 疾病相关Defects in FZD4 are the cause of vitreoretinopathy exudative type 1 (EVR1) [MIM:133780]; also known as autosomal dominant familial exudative vitreoretinopathy (FEVR) or Criswick-Schepens syndrome. EVR1 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery.
  • 序列相似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 结构域Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 344 antibody
    • CD 344 antigen antibody
    • CD344 antibody
    • CD344 antigen antibody
    • EVR1 antibody
    • FEVR antibody
    • Frizzled (Drosophila) homolog 4 antibody
    • Frizzled 4 antibody
    • Frizzled family receptor 4 antibody
    • Frizzled homolog 4 (Drosophila) antibody
    • Frizzled-4 antibody
    • Fz 4 antibody
    • Fz-4 antibody
    • Fz4 antibody
    • FZD4 antibody
    • FZD4_HUMAN antibody
    • FZD4S antibody
    • FzE4 antibody
    • GPCR antibody
    • hFz4 antibody
    • MGC34390 antibody
    • WNT receptor frizzled 4 antibody
    see all

Anti-Frizzled 4 antibody 图像

  • Anti-Frizzled 4 antibody (ab77724) at 1 µg/ml + Small Intestine (Ileum) cell lysate at 35 µg

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

Anti-Frizzled 4 antibody (ab77724)参考文献

This product has been referenced in:
  • Formosa A  et al. MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 locus, regulate proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 24166498) »

See 1 Publication for this product

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