概述

  • 产品名称Anti-Frizzled 2抗体
    参阅全部 Frizzled 2 一抗
  • 描述
    兔多克隆抗体to Frizzled 2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from the N-terminal region of human FZD2.

  • 阳性对照
    • Human PC-3, SKOV3, SW480 and HEK293 cell lysates for Western blotting and HepG2 for immunoprecipitation.

性能

应用

Our Abpromise guarantee covers the use of ab52565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 63 kDa). The observed molecular weight of ~ 85 kDa may be due to post-translational modifications such as glycosylation, which is predicted in the amino acid sequence.
IP Use at an assay dependent dilution. Use at a concentration of 7 µg/reaction.

靶标

  • 功能Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 组织特异性Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.
  • 序列相似性Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 结构域Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Frizzled homolog 2 antibody
    • Frizzled-2 antibody
    • Frizzled2 antibody
    • Fz 2 antibody
    • Fz-2 antibody
    • Fz2 antibody
    • FZD 2 antibody
    • FZD2 antibody
    • FZD2_HUMAN antibody
    • FzE 2 antibody
    • FzE2 antibody
    • hFz 2 antibody
    • hFz2 antibody
    • Mfz10 antibody
    see all

Anti-Frizzled 2 antibody (ab52565)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for your enquiry regarding ab52565.

This antibody has not been tested for functional or any blocking assay. As the storage buffer contains 0.1% sodium azide which is a cytotoxic agent thus ab52565 is unsuitable for cell culture &quo...

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Thank you for your inquiry and I sincerely apologize for the delay as I was awaiting more information from the supplying laboratory. The immunogen is a peptide derived from N-terminal region. We can say that the immunogen is in the first 75 amino ac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"