Free Fatty Acid Uptake Assay试剂盒(Fluorometric) (ab176768)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells
概述
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产品名称
Free Fatty Acid Uptake Assay试剂盒(Fluorometric) -
检测方法
Fluorescent -
样品类型
Adherent cells -
检测类型
Cell-based (quantitative) -
检测时间
1h 00m -
种属反应性
与反应: Mammals, Other species -
产品概述
Free Fatty Acid Uptake Assay Kit (Fluorometric) (ab176768) provides a simple and sensitive method for the measurement of fatty acid uptake in cells containing fatty acid transporters. The kit uses a proprietary dodecanoic acid fluorescent fatty acid substrate. This fatty acid uptake assay kit can be performed on any fluorescence microplate reader with a bottom-read mode at Ex/Em = 485/515 nm or FITC channel. The assay can be performed in 96-well or 384-well microtiter plates in a simple mix-and-read procedure, and easily adapted for high throughput screening applications.
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说明
Fatty acid uptake is an important therapeutic target for the treatment of many human diseases such as obesity, type 2 diabetes and hepatic steatosis.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer 1 x 10ml DMSO 1 x 100µl TF2-C12 Fatty Acid 1 vial -
研究领域
图片
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Cells were plated at 50,000 cells/100μL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37 ºC, 5% CO2 incubator for 30 min. At the end of the incubation time, 100 μL of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a plate reader using bottom read mode. A – fibroblasts (Control); B – fibroblasts (Insulin); C – adipocytes (Control); D– adipocytes (Insulin).
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab176768 被引用在 21 文献中.
- Yoo A et al. Neuronal growth regulator 1 promotes adipocyte lipid trafficking via interaction with CD36. J Lipid Res 63:100221 (2022). PubMed: 35526561
- Rao Y et al. A novel HSF1 activator ameliorates non-alcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation. Br J Pharmacol 179:1411-1432 (2022). PubMed: 34783017
- Buettner R et al. Targeting the metabolic vulnerability of acute myeloid leukemia blasts with a combination of venetoclax and 8-chloro-adenosine. J Hematol Oncol 14:70 (2021). PubMed: 33902674
- Liu S et al. S100A4 enhances protumor macrophage polarization by control of PPAR-?-dependent induction of fatty acid oxidation. J Immunother Cancer 9:N/A (2021). PubMed: 34145030
- Yang Y et al. SLC25A1 promotes tumor growth and survival by reprogramming energy metabolism in colorectal cancer. Cell Death Dis 12:1108 (2021). PubMed: 34839347