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Abcam's Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)
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This assay detects formation of C-8 (octanoate) and longer fatty acids.
|ACS Reagent||Blue||1 vial|
|Enhancer||Purple||1 x 200µl|
|Fatty Acid Assay Buffer||WM||1 x 25ml|
|Fatty Acid enzyme mix||Green||1 vial|
|Fatty Acid Probe (in DMSO)||Red||1 x 200µl|
|Palmitic Acid Standard (1nmol/µl)||Yellow||1 x 300µl|
Our Abpromise guarantee covers the use of ab65341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Free Fatty Acid measured in biologicals showing concentration (µM)
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted