概述

  • 产品名称Anti-FPRL1抗体[GM1D6]
    参阅全部 FPRL1 一抗
  • 描述
    小鼠单克隆抗体[GM1D6] to FPRL1
  • 特异性Ab26316 recognises FPRL1 transiently expressed on the cell surface of transfected BOSC cells.
  • 经测试应用适用于: IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Horse, Human
  • 免疫原

    Ab26316 was generated by genetic immunisation of BALB/c mice with a proprietary GPCR-immunisation vector containing the FPRL1 cDNA.

性能

应用

Our Abpromise guarantee covers the use of ab26316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22384219
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • 组织特异性Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALXR antibody
    • FMLP-R-I antibody
    • FMLP-R-II antibody
    • FMLP-related receptor I antibody
    • FMLPX antibody
    • Formyl peptide receptor 2 antibody
    • Formyl peptide receptor related antibody
    • Formyl peptide receptor-like 1 antibody
    • FPR2 antibody
    • FPR2_HUMAN antibody
    • FPR2A antibody
    • FPRH1 antibody
    • FPRH2 antibody
    • FPRL1 antibody
    • HM63 antibody
    • Lipoxin A4 receptor antibody
    • LXA4 receptor antibody
    • LXA4R antibody
    • N-formyl peptide receptor 2 antibody
    • RFP antibody
    see all

Anti-FPRL1 antibody [GM1D6] 图像

  • ab26316 staining of FPRL1 in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1 and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

  • FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1 (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.
  • ab26316, staining FPRL1 (green) in and sub-acutely injured equine superficial digital flexor tendons by Immunohistochemistry (Frozen sections).

    After warming cryosections at room temperature for 30 minutes, sections were rehydrated in 1% PBS-Tween 20 (PBS-T) and blocked in PBS containing 5% normal goat serum for 1 hour at room temperature. Sections were incubated with the primary antibody (1/100 in PBS + 5% normal goat serum) for 2 hours. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/300).

Anti-FPRL1 antibody [GM1D6] (ab26316)参考文献

This product has been referenced in:
  • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »
  • Dakin SG  et al. Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair. PLoS One 7:e32333 (2012). IHC-Fr ; Horse . Read more (PubMed: 22384219) »

See all 4 Publications for this product

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The antibody ab26316 recognizes the extracellular part of the receptor. We tested this in FACS on transiently transfected cells. Some customers tested the antibody and its ability ...

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Antibodies are sometimes pre-adsorbed. This means they have been adsorbed with other proteins, or serum from various species, etc, to eliminate any antibody that may cross-react. The resulting purified antibody s...

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