概述

  • 产品名称
  • 描述
    兔多克隆抗体to FPR1
  • 特异性
    ab113531 detects endogenous levels of total FPR1 protein.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, from an internal region of Human FPR1.

  • 阳性对照
    • Human spleen tissue; K562 cell extracts; MCF7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab113531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    High affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • 组织特异性
    Neutrophils.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    Phosphorylated; which is necessary for desensitization.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • fMet Leu Phe receptor antibody
    • fMet-Leu-Phe receptor antibody
    • FMLP antibody
    • fMLP receptor antibody
    • FMLPR antibody
    • Formyl peptide receptor 1 antibody
    • FPR 1 antibody
    • FPR antibody
    • FPR receptor antibody
    • FPR1 antibody
    • FPR1_HUMAN antibody
    • N formyl peptide chemoattractant receptor antibody
    • N formyl peptide receptor antibody
    • N formylpeptide chemoattractant receptor antibody
    • N-formyl peptide receptor antibody
    • N-formylpeptide chemoattractant receptor antibody
    see all

图片

  • All lanes : Anti-FPR1 antibody (ab113531) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : K562 cell extract with immunizing peptide


    Predicted band size : 38 kDa
  • ab113531 at 5ug/ml staining FPR1 in Formalin-Fixed, Paraffin-Embedded Human spleen tissue by Immunohistochemistry.
  • ab113531 at a 1/100 dilution staining FPR1 in Human MCF7 cells by Immunofluorescence. The cells on the right were incubated with the immunizing peptide.

文献

This product has been referenced in:

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Jul 19 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply.

I have confirmed with the laboratory that we have not tested the antibody for cross-reactivity with FPR2. However, I compared the sequence of the peptide used to generate ab113531 to the corresponding region of FPR2...

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Thank you for contacting Abcam regarding ab113531.

Regarding the molecular weight of the FPR1 protein, the predicted molecular weight is indeed 38kDa, however this protein is also subject to several posttranslational modifications, including...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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