A region within synthetic peptide: PFPQPPQKDE GSNCSEDKVV ESLPSSSSEQ SPLQKQGIHS PSDFELTEEE, corresponding to internal sequence amino acids 109-158 of Human FOXR2
- Jurkat cell lysate; human kidney tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
Anti-FOXR2 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXR2 antibody (ab55961)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXR2 with ab55961 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
Western blot - FOXR2 antibody (ab55961)
Lane 1 :
markerLane 2 :
Anti-FOXR2 antibody (ab55961) at 1.25 µg/mlLane 1 :
As aboveLane 2 :
Jurkat cell lysate at 10 µgSecondaryLane 2 :
HRP-labelled anti-rabbit IgG at 1/50000 dilutionPredicted band size :
36 kDaObserved band size :
28 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
Anti-FOXR2 antibody (ab55961)参考文献
has not yet been referenced specifically in any publications.
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