概述

  • 产品名称Anti-FOXQ1抗体
    参阅全部 FOXQ1 一抗
  • 描述
    兔多克隆抗体to FOXQ1
  • 经测试应用适用于: ELISA, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: GDDSLGSDGD CAANSPAAGG GARDPPGDGE QSAGGGPGAE EAIPAAAAAA, corresponding to N terminal amino acids 36-85 of Human FOXQ1

  • 阳性对照
    • Jurkat cell lysate and human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab51340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 组织特异性Expressed predominantly in the stomach, trachea, bladder and salivary gland.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Forkhead box protein Q1 antibody
    • Forkhead box Q1 antibody
    • FOX Q1 antibody
    • FOXQ 1 antibody
    • FOXQ1 antibody
    • FOXQ1_HUMAN antibody
    • Hepatocyte nuclear factor 3 forkhead antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 1 antibody
    • HFH 1 antibody
    • HFH-1 antibody
    • HFH1 antibody
    • HNF 3/forkhead like protein 1 antibody
    • HNF-3/forkhead-like protein 1 antibody
    • HNF3/forkhead like protein 1 antibody
    • MGC77469 antibody
    • Q1 antibody
    • Winged helix / forkhead transcription factor antibody
    • Winged helix/forkhead transcription factor antibody
    • zgc:77469 antibody
    see all

Anti-FOXQ1 antibody 图像

  • Anti-FOXQ1 antibody (ab51340) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXQ1 with ab51340 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells of renal tubule. Magnification: 400X.

  • ICC/IF image of ab51340 stained cells. The cells were and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FOXQ1 antibody (ab51340)参考文献

This product has been referenced in:
  • Xiang XJ  et al. MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1. Cell Physiol Biochem 36:1382-94 (2015). WB ; Human . Read more (PubMed: 26159618) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"