Anti-FOXP3抗体[mAbcam 22510] (ab22510)

概述

  • 产品名称
    Anti-FOXP3抗体[mAbcam 22510]
    参阅全部 FOXP3 一抗
  • 描述
    小鼠单克隆抗体[mAbcam 22510] to FOXP3
  • 宿主
    Mouse
  • 特异性
    This product detects an epitope near the C-terminus of the FOXP3 protein and is expected to detect full length FOXP3. It is not expected to detect the cleaved forms of FOXP3.
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide derived from residues 400 to the C-terminus of Human FOXP3.

  • 阳性对照
    • Tonsil.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab22510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FOXP3 peptide (ab16809).

靶标

  • 功能
    Probable transcription factor. Plays a critical role in the control of immune response.
  • 疾病相关
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • 序列相似性
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

图片

  • ab22510 staining cultured rat OCPs by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized 30 minutes with 0.1% PBS-0.3% TritonX and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab22510 at 1/1000 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue.

    See Abreview

  • All lanes : Anti-FOXP3 antibody [mAbcam 22510] (ab22510) at 1 µg/ml

    Lane 1 : HEK293 cells overexpressing human FOXP3
    Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)


    Exposure time: 45 seconds
  • Clone mAbcam 22510 used to stain formalin-fixed paraffin-embedded sections of human tonsil. Antigens were heat retrieved with pH9 Tris-EDTA buffer.

    These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).

  • IHC image of ab22510 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22510, 20µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Cooks T  et al. Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246. Nat Commun 9:771 (2018). Read more (PubMed: 29472616) »
  • Ma A  et al. Anti-inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells. Mol Med Rep 15:3615-3622 (2017). Read more (PubMed: 28440447) »

See all 49 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Oct 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (OPC culture)
Specification
OPC culture
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 13 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, Embryonic body)
Loading amount
20 µg
Specification
Brain, Embryonic body
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (non-Hodgkin and Hoddgkin lymphoma tissue microarra)
Specification
non-Hodgkin and Hoddgkin lymphoma tissue microarra
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Username

Dr. Alexandar Tzankov

Verified customer

提交于 Dec 06 2006

Product Anti-FOXP3 antibody [mAbcam 22510] (ab22510) is a mouse monoclonal Ab of isotype IgG1; a suitable isotype control for this primary would be: ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Username

Unsong Oh

Verified customer

提交于 Sep 16 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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