概述

  • 产品名称
  • 描述
    兔多克隆抗体to FOXP3
  • 特异性
    This antibody has shown reactivity against mouse protein (CD4+CD25+ T cells). There appears to be no reactivity against human Jurkat and mouse CD4+CD25- T cell lysates. Other species and samples have not been tested.
  • 经测试应用
    适用于: IP, IHC-Fr, IHC-P, WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Mouse FOXP3 Gly43 to His100 peptide and Flag Tag fused at C terminal of tag protein.

  • 阳性对照
    • Mouse CD4+CD25+ T cells

性能

应用

Our Abpromise guarantee covers the use of ab54501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Flow Cyt 1/8000. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

靶标

  • 功能
    Probable transcription factor. Plays a critical role in the control of immune response.
  • 疾病相关
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • 序列相似性
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

图片

  • All lanes : Anti-FOXP3 antibody (ab54501) at 1/2000 dilution

    Lane 1 : Jurkat Tag cells transfected with the expression vector
    Lane 2 : Jurkat Tag cells transfected with the FOXP3 expression construct


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.24 hours after transfection, cells were lysed and analyzed by Western blot using ab54501
  • ab54501 staining FOXP3 in Mouse thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in 1% BSA/PBS) for 1 hour at 25°C. An undiluted HRP-conjugated Goat polyclonal was used as the secondary antibody.

    See Abreview

  • ab54501 staining FOXP3 in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 5% serum) for 24 hours at 4°C. ab150064, a donkey anti-rabbit Alexa Fluor® 594 (IgG H&L, 1/1000) was used as the secondary antibody.

    See Abreview

  • ab54501(10µg/ml) staining FOXP3 (red) in paraffin-embedded Mouse spleen sections. Tissue was fixed in paraformaldehyde, blocking performed using 5% serum (30 mins/25oC) and heat mediated antigen retrieval performed before staining. For further experimental details please refer to abreview.

    See Abreview

  • ab54501 at 1/500 dilution staining 1) vector and 2) FOXP3 transfected Jurkat tag cells; visualised with Cy3 conjugated secondary antibody. Magnification: x100
  • ab54501 at 1/500 dilution staining Jurkat Tag cells transfected with 1) the expression vector and 2) the FOXP3 expression construct visualised with HRPO conjugated secondary antibody. Magnification: x100
  • ab54501 at 1/8000 dilution staining Jurkat Tag cells transfected with 1) the expression vector and 2) the FOXP3 expression construct with FITC conjugated secondary antibody. Percentages of positively stained cells are shown.

文献

This product has been referenced in:
  • Chen S  et al. PD-L1 expression of the residual tumor serves as a prognostic marker in local advanced breast cancer after neoadjuvant chemotherapy. Int J Cancer 140:1384-1395 (2017). IHC-P ; Human . Read more (PubMed: 27925176) »
  • Roth-Walter F  et al. Janus-faced Acrolein prevents allergy but accelerates tumor growth by promoting immunoregulatory Foxp3+ cells: Mouse model for passive respiratory exposure. Sci Rep 7:45067 (2017). Read more (PubMed: 28332605) »

See all 19 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Thymus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6
Permeabilization
No
Specification
Thymus
Blocking step
in the kit : Ultratek HRP (anti-polyvalent) ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumro xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Permeabilization
No
Specification
tumro xenograft
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumour)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Tumour
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Jim Manavis

Verified customer

提交于 Aug 16 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Lymph Node
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Jim Manavis

Verified customer

提交于 Aug 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Thymus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6.0
Permeabilization
No
Specification
Thymus
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 24 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Methanol
Username

Ms. CM ou

Verified customer

提交于 May 14 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
solution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample
Mouse Tissue sections (breast cancer tumor)
Specification
breast cancer tumor
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. R Berahovich

Verified customer

提交于 Aug 12 2013

Triton X-100 is the most common permeabilizing agent used for nuclear staining. With ab54501, the lab uses the following permeabilization protocol after PFA fixation of the cells:

Permeabilize cells with in 0.2% Triton X-100 plus 1% normal se...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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I am sorry that the staining using anti-CD4 antibody (ab51312) and anti-FOXP3 antibody (ab54501) have not been as you have expected.

With all of our products, we advise that our customers are likely to need to optimise the conditions used in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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