概述

  • 产品名称Anti-FOXP3抗体
    参阅全部 FOXP3 一抗
  • 描述
    兔多克隆抗体to FOXP3
  • 特异性For Western Blot, this antibody has been tested in HEK293 overexpressing mouse FOXP3, but has not been tested against endogenous FOXP3.
  • 经测试应用适用于: WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse FOXP3.

    .

  • 阳性对照
    • HEK293 overexpressing mouse FOXP3
  • 常规说明


    This antibody specifically recognises mouse FOXP3 and does not recognise human FOXP3.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab15590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).Can be blocked with Mouse FOXP3 peptide (ab15736).
IHC-FoFr 1/300.

靶标

  • 功能Probable transcription factor. Plays a critical role in the control of immune response.
  • 疾病相关Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • 序列相似性Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

Anti-FOXP3 antibody 图像

  • Immunohistochemistical detection of FOXP3 using antibody ab15590 on paraformaldehyde perfusion fixed mouse heart tissue sections. Primary antibody  ab15590 diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit biotin conjugated (1/300). Immunoreactivity visualised by DAB; the antibody produced a puctuated and cytoplasmic staining in cardiac cells.

    See Abreview

Anti-FOXP3 antibody (ab15590)参考文献

This product has been referenced in:
  • Barbarulo A  et al. Notch3 and canonical NF-kappaB signaling pathways cooperatively regulate Foxp3 transcription. J Immunol 186:6199-206 (2011). WB ; Mouse . Read more (PubMed: 21508258) »
  • Fragale A  et al. IFN regulatory factor-1 negatively regulates CD4+ CD25+ regulatory T cell differentiation by repressing Foxp3 expression. J Immunol 181:1673-82 (2008). Flow Cyt ; Mouse . Read more (PubMed: 18641303) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen and Lymphnode)
Specification Spleen and Lymphnode
Fixative Acetone
Permeabilization Yes - 0.25% TritonX 10min RT
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 17 2011

Thank you for your patience while we waited for a response from the laboratory. I have heard back from the laboratory and there is an error on the public website on the applications that this antibody has been tested in. The antibody ab15590, F...

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Thank you for contacting Abcam about ab15590 (FOXP3). I have sent your enquiry along to our laboratory for clarification but I can tell you that based on the information on the data sheet on our public website this antibody should work in IHC-P (p...

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Thank you for your enquiry. I would suggest blocking the non-specific binding sites with 5% BSA in TBS-T for 1 hr at room temperature. It may be worth using this blocking solution for diluting the primary and the secondary antibody as well. You ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 18 2010

Thank you for your enquiry. The minor bands that you see on the western blot image are non-specific bands, and are seen in all the lanes. Some of the common reasons why non-specific bands are detected maybe due to the primary, and or secondary antib...

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Thank you for contacting us for technical support and providing the details of the customer's protocol. The antibody ab15590 has not been tested against endogenous levels of FOXP3, only on HEK293 overexpressing mouse FOXP3 and did not work against h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"