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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse FOXP3.
This antibody specifically recognises mouse FOXP3 and does not recognise human FOXP3.
Our Abpromise guarantee covers the use of ab15590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).Can be blocked with Mouse FOXP3 peptide (ab15736).|
Immunohistochemistical detection of FOXP3 using antibody ab15590 on paraformaldehyde perfusion fixed mouse heart tissue sections. Primary antibody ab15590 diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit biotin conjugated (1/300). Immunoreactivity visualised by DAB; the antibody produced a puctuated and cytoplasmic staining in cardiac cells.
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