概述

  • 产品名称
  • 描述
    兔多克隆抗体to FOXP3
  • 特异性
    This antibody recognises a single clean band representing FOXP3 on HEK293 cell lysate overexpressing human FOXP3. This band is blocked by the immunising peptide and is not present in the control HEK293 cell lysate.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human FOXP3.

    .

  • 阳性对照
    • This antibody gave a positive signal in Hek 293 lysate over expressing human FOXP3

性能

应用

Our Abpromise guarantee covers the use of ab10901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human FOXP3 peptide (ab16809).
IHC-P Use a concentration of 0.3 - 1 µg/ml.
ICC/IF Use a concentration of 2 - 4 µg/ml.
ChIP Use at an assay dependent concentration.

靶标

  • 功能
    Probable transcription factor. Plays a critical role in the control of immune response.
  • 疾病相关
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • 序列相似性
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

图片

  • All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/ml

    Lane 1 : 20µg HEK 293 lysate
    Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3
    Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3
    Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Secondary
    Lanes 4 - 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml


    Performed under reducing conditions.

    Predicted band size : 47 kDa


    Exposure time : 1 minute

    A no primary control lane showed no bands.

文献

This product has been referenced in:
  • Shimizu C  et al. The role of TGF-ß and myofibroblasts in the arteritis of Kawasaki disease. Hum Pathol 44:189-98 (2013). Read more (PubMed: 22955109) »
  • Mehra S  et al. The Mycobacterium tuberculosis stress response factor SigH is required for bacterial burden as well as immunopathology in primate lungs. J Infect Dis 205:1203-13 (2012). IHC ; Non human primates . Read more (PubMed: 22402035) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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