概述

  • 产品名称Anti-FOXP3抗体
    参阅全部 FOXP3 一抗
  • 描述
    兔多克隆抗体to FOXP3
  • 特异性This antibody recognises a single clean band representing FOXP3 on HEK293 cell lysate overexpressing human FOXP3. This band is blocked by the immunising peptide and is not present in the control HEK293 cell lysate.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human FOXP3.

    .

  • 阳性对照
    • This antibody gave a positive signal in Hek 293 lysate over expressing human FOXP3

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab10901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human FOXP3 peptide (ab16809).
IHC-P Use a concentration of 0.3 - 1 µg/ml.
ICC/IF Use a concentration of 2 - 4 µg/ml.
ChIP Use at an assay dependent concentration.

靶标

  • 功能Probable transcription factor. Plays a critical role in the control of immune response.
  • 疾病相关Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • 序列相似性Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

Anti-FOXP3 antibody 图像

  • All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/ml

    Lane 1 : 20µg HEK 293 lysate
    Lane 2 : 20µg HEK 293 lysate over expressing human FOXP3
    Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3
    Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml
    Lane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

    Secondary
    Lanes 4 - 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml


    Performed under reducing conditions.

    Predicted band size : 47 kDa


    Exposure time : 1 minute

    A no primary control lane showed no bands.

Anti-FOXP3 antibody (ab10901)参考文献

This product has been referenced in:
  • Mehra S  et al. The Mycobacterium tuberculosis stress response factor SigH is required for bacterial burden as well as immunopathology in primate lungs. J Infect Dis 205:1203-13 (2012). IHC ; Non Human Primates . Read more (PubMed: 22402035) »
  • Oh U  et al. Reduced Foxp3 Protein Expression Is Associated with Inflammatory Disease during Human T Lymphotropic Virus Type 1 Infection. J Infect Dis 193:1557-66 (2006). Read more (PubMed: 16652285) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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提交于 Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"