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Our Abpromise guarantee covers the use of ab36607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.