Anti-FOXP2抗体- C-terminal (ab1307)

概述

  • 产品名称
    Anti-FOXP2抗体- C-terminal
    参阅全部 FOXP2 一抗
  • 描述
    山羊多克隆抗体to FOXP2 - C-terminal
  • 特异性
    This antibody is expected to recognise all three reported isoforms (NP_055306.1; NP_683696.2; NP_683697.1).
  • 经测试应用
    适用于: WB, IHC-P, IHC - Wholemount, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Zebra finch
    预测可用于: Rat, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human FOXP2 aa 703-715 (C terminal) (Cysteine residue).
    Sequence:

    C-REIEEEPLSEDLE


    Database link: O15409
    (Peptide available as ab22800)

  • 阳性对照
    • WB: Whole tissue lysate from human cerebellum. IHC-Fr: e12.5 mouse embryonic spinal cord tissue. IHC-P: Human testis tissue. ICC/IF: Mouse e12.5 thoracic spinal cord cells. IHC-wholemount: Mouse brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab1307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79.9 kDa).Can be blocked with Human FOXP2 peptide (ab22800).
IHC-P Use at an assay dependent concentration. PubMed: 16901978
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23967072
IHC-Fr Use at an assay dependent concentration. PubMed: 20484636

靶标

  • 功能
    Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
  • 组织特异性
    Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
  • 疾病相关
    Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
    Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
  • 序列相似性
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 发展阶段
    Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
  • 结构域
    The leucine-zipper is required for dimerization and transcriptional repression.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAG repeat protein 44 antibody
    • CAGH44 antibody
    • DKFZp686H1726 antibody
    • Forkhead box P2 antibody
    • Forkhead box protein P2 antibody
    • forkhead/winged-helix transcription factor antibody
    • FOX P2 antibody
    • FOXP2 antibody
    • FOXP2_HUMAN antibody
    • HGNC11222 antibody
    • HGNC11956 antibody
    • SPCH 1 antibody
    • SPCH1 antibody
    • TNRC 10 antibody
    • TNRC10 antibody
    • trinucleotide repeat containing 10 antibody
    • Trinucleotide repeat containing gene 10 protein antibody
    • Trinucleotide repeat-containing gene 10 protein antibody
    see all

图片

  • Paraffin-embedded human testis tissue stained for FOXP2 with ab1307 at 5 µg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6. AP staining.

  • ab1307 staining FOXP2 in e12.5 mouse embryonic spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 30 minutes at 20°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 16 hours at 4°C. The secondary antibody used was a donkey anti-sheep (and goat) IgG conjugated to Alexa Fluor® 594 (red) used at a 1/2000 dilution.

    See Abreview

  • Anti-FOXP2 antibody - C-terminal (ab1307) at 0.5 µg/ml + Whole tissue lysate from human cerebellum (35µg protein in RIPA buffer)

    Predicted band size : 79.9 kDa
  • Mouse brain tissue stained for FOXP2 with ab1307 (1/1000 dilution) in immunohistochemical analysis.

    Brain explant taken from E15 embryo and cultured for 1 month. Fixed for 4 hours in 4% PFA, permeabilised with PBS-TritonX 100 (0,25%). FOXP2 antibody (ab1307) was used at 1/1000 and a monoclonal mouse antibody was used to visualize CaBP. Secondary antibody incubated at RT for 2 hours.
    Image shows and overlay of FoxP2-positive deep cerebellar nuclei neurons (red) and CaBP-positive Purkinje cells (green).

    See Abreview

  • Mouse e12.5 thoracic spinal cord stained for FOXP2 (green) using ab1307 (1/2000 dilution) in ICC/IF.

文献

This product has been referenced in:
  • Vicario A  et al. Genoarchitecture of the extended amygdala in zebra finch, and expression of FoxP2 in cell corridors of different genetic profile. Brain Struct Funct 222:481-514 (2017). ICC/IF ; Zebra finch . Read more (PubMed: 27160258) »
  • Kabayiza KU  et al. The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development. Front Mol Neurosci 10:157 (2017). ICC/IF ; Mouse . Read more (PubMed: 28603487) »

See all 12 Publications for this product

客户评价及客户问答

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain
Username

Ms. Lea Hachigian

Verified customer

提交于 Jul 23 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Mouse Cell lysate - whole cell (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Brain explant)
Specification
Brain explant
Username

Abcam user community

Verified customer

提交于 Sep 28 2012

That's my pleasure.

If you have any further questions in the future please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you forcontacting us. I am sorry to hear that this antibody is not providing satisfactory results.

Theuse of this antibody inIHC-Frwasfirst describedby one of our customersand his result andprotocol can be foundon the Abreview: http://...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e12.5 mouse embryonic spinal cord)
Specification
e12.5 mouse embryonic spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Frederic Clotman

Verified customer

提交于 Sep 17 2010

Thank you for your email. As soon as we receive new information regarding our products, we update the online datasheets. So keep an eye on the datasheets for more information. Also, we're always adding new products, and I do know that we will be adding...

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Thank you for your email. I have checked with the originator of this antibody who has provided me with the following information regarding your question. It seems that since the design of Ab1307, more members of the FOXP family have been identified,...

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Thank you for your phone call. Ab1307 has not been tested yet for cross-reactivity with other FOXP family members. However, based on a BLAST search with the immunizing peptide (REIEEEPLSEDLE) we would not expect it to be a problem. If you have any ...

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