概述

  • 产品名称Anti-FOXP2抗体
    参阅全部 FOXP2 一抗
  • 描述
    兔多克隆抗体to FOXP2
  • 经测试应用适用于: WB, IHC-FoFr, IHC-Fr, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human FOXP2.

    (Peptide available as ab16278.)

  • 阳性对照
    • This antibody gave a positive signal in HEK293 Whole Cell Lysate IHC-P: FFPE mouse foetal e17 brain tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab16046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Can be blocked with Human FOXP2 peptide (ab16278). (see abreview)
IHC-FoFr Use at an assay dependent concentration. PubMed: 19490899
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/2500. PubMed: 19136970

靶标

  • 功能Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
  • 组织特异性Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
  • 疾病相关Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
    Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
  • 序列相似性Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 发展阶段Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
  • 结构域The leucine-zipper is required for dimerization and transcriptional repression.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAG repeat protein 44 antibody
    • CAGH44 antibody
    • DKFZp686H1726 antibody
    • Forkhead box P2 antibody
    • Forkhead box protein P2 antibody
    • forkhead/winged-helix transcription factor antibody
    • FOX P2 antibody
    • FOXP2 antibody
    • FOXP2_HUMAN antibody
    • HGNC11222 antibody
    • HGNC11956 antibody
    • SPCH 1 antibody
    • SPCH1 antibody
    • TNRC 10 antibody
    • TNRC10 antibody
    • trinucleotide repeat containing 10 antibody
    • Trinucleotide repeat containing gene 10 protein antibody
    • Trinucleotide repeat-containing gene 10 protein antibody
    see all

Anti-FOXP2 antibody 图像

  • IHC image of FOXP2 staining in mouse e17 foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16046, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • This image is courtesy of an anonymous Abreview

    ab16046 at 1/1000 detecting FOXP2 from human 293T cell lysate (whole cell) (60ug/lane) by Western Blot. An HRP conjugated goat anti-rabbit IgG was used as the secondary and ECL was used as the detection method (1 minute exposure).

    See Abreview

  • Mouse spinal cord was fixed in paraformaldehyde, blocked in 1% BSA for 30 minutes then incubated with ab16046 at 1/8000 dilution for 18 hours. This image was submitted as part of a review by Jeremy Dasen.

  • Image courtesy of Human Protein Atlas

    ab16046 staining FOXP2 in human testis. Paraffin embedded human testis tissue was incubated with ab16046 (1/600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16046 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • Anti-FOXP2 antibody (ab16046) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab16046 staining FOXP2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde, permeabilized by 0.4% Triton X and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/8000) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.

    See Abreview

  • ICC/IF image of ab16046 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FOXP2 antibody (ab16046)参考文献

This product has been referenced in:
  • Houlihan SL  et al. Upregulation of neurovascular communication through filamin abrogation promotes ectopic periventricular neurogenesis. Elife 5:N/A (2016). IHC-Fr ; Mouse . Read more (PubMed: 27664421) »
  • Vong KI  et al. Sox9 is critical for suppression of neurogenesis but not initiation of gliogenesis in the cerebellum. Mol Brain 8:25 (2015). Read more (PubMed: 25888505) »

See all 38 Publications for this product

Product Wall

Application Western blot
Sample Zebra finch Recombinant protein (Hek overexpressing zebra finch FoxP2)
Loading amount 10 µg
Specification Hek overexpressing zebra finch FoxP2
Gel Running Conditions Reduced Denaturing (5% Tris-Glycine)
Blocking step Roti Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 15 2013

Thank you for your enquiry.

Perhaps it would be helpful if you could forward the western blot image along as an attachement?

Thanks for the additional information!

That's my pleasure.

If you have any further questions in the future please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E15.5 brain)
Specification E15.5 brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Nam-Ho Kim

Verified customer

提交于 May 21 2012

Thank you for contacting Abcam. The immunogen sequence for ab16046 is (C)*******************. If there is anything else I can help you with, please let me kn

Thank you for providing that extra information and informing us of the problems you have been having with ab16046. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Unfortunately,...

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Thank you for contacting us. In answer to your enquiry, the lysis buffer used to prepare the sample of HEK293 cells was not exactly as from the protocol you have found on our website but was purchased from one of the larger reagent suppliers. The compo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Catarina Ramos

Verified customer

提交于 Jan 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.4% Triton X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 14 2010

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