概述

  • 产品名称
    Anti-FOXP1抗体[JC12]
    参阅全部 FOXP1 一抗
  • 描述
    小鼠单克隆抗体[JC12] to FOXP1
  • 特异性
    This antibody does not recognise closely related molecules FOXP2, FOXP3 or FOXP4.
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human, Zebra finch
  • 免疫原

    Full length native protein (purified) of Mouse FOXP1. JC12 is thought to be a mouse autoantibody.

  • 阳性对照
    • WB: BCBL1 human cell line or Mutul human cell line. IHC-P: Human Hodgkin's lymphoma and human colon tissues.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

应用

Our Abpromise guarantee covers the use of ab32010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 20145110
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500. Predicted molecular weight: 75 kDa.

靶标

  • 功能
    Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
  • 疾病相关
    Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
    Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
  • 序列相似性
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 结构域
    The leucine-zipper is required for dimerization and transcriptional repression.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 12CC4 antibody
    • FLJ23741 antibody
    • Fork head related protein like B antibody
    • Forkhead box P1 antibody
    • Forkhead box protein P1 antibody
    • FOX P1 antibody
    • FOXP 1 antibody
    • foxp1 antibody
    • FOXP1_HUMAN antibody
    • Glutamine rich factor 1 antibody
    • hFKH1B antibody
    • HSPC215 antibody
    • MGC12942 antibody
    • MGC88572 antibody
    • MGC99551 antibody
    • QRF 1 antibody
    • QRF1 antibody
    see all

Anti-FOXP1 antibody [JC12] 图像

  • IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

    The inset negative control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

     

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HEK293 Whole Cell Lysate Overexpressing FOXP1

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

    Predicted band size : 75 kDa
    Observed band size : 80,100 kDa (why is the actual band size different from the predicted?)
  • IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

    The inset negative control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

     

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution

    Lane 1 : BCBL1 human cell line
    Lane 2 : Mutul human cell line


    Predicted band size : 75 kDa
    Observed band size : 60,65,80 kDa (why is the actual band size different from the predicted?)
    This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5): 2816-24.

    Image kindly provided by H. Hu.

Anti-FOXP1 antibody [JC12] (ab32010)参考文献

This product has been referenced in:

See all 18 Publications for this product

Product Wall

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (5% Tris-Glycine)
Sample
Zebra finch Cell lysate - whole cell (Hek overexpressing zebra finch FoxP1)
Specification
Hek overexpressing zebra finch FoxP1
Blocking step
Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 14 2013

Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending two free of charge replacements that should arrive tomorrow on the order ***. Please keep me updated on how these work in ...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Specification
brain tissue
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
Yes - PBS-T
Username

Abcam user community

Verified customer

提交于 May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
60 µg
Specification
293T
Treatment
transfected with EGFP FOXP 1
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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