Anti-FOXP1抗体(ab16645)
Key features and details
- Rabbit polyclonal to FOXP1
- Suitable for: IHC-FoFr, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FOXP1抗体
参阅全部 FOXP1 一抗 -
描述
兔多克隆抗体to FOXP1 -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab16645 are tested in IHC-P. Previous batches were additionally validated in IHC-FoFr and WB. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab134055.
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经测试应用
适用于: IHC-FoFr, IHC-P, WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Chicken, Dog, Human, Xenopus laevis -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-p: Mouse brain
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16645于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-FoFr |
Use at an assay dependent concentration.
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IHC-P | (4) |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (7) |
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details). |
说明 |
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IHC-FoFr
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details). |
靶标
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功能
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
疾病相关
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
序列相似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
结构域
The leucine-zipper is required for dimerization and transcriptional repression. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 416092 Chicken
- Entrez Gene: 484692 Dog
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Entrez Gene: 496386 Xenopus laevis
- Entrez Gene: 779033 Xenopus laevis
- Omim: 605515 Human
see all -
别名
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
图片
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Paraffin-embedded mouse brain tissue stained for FOXP1 using ab16645 in immunohistochemical analysis.
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All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution
Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity) -
ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
数据表及文件
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SDS download
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Datasheet download
文献 (97)
ab16645 被引用在 97 文献中.
- Limone F et al. Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cues. Cell Rep 42:111896 (2023). PubMed: 36596304
- Liau ES et al. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14:46 (2023). PubMed: 36596814
- Wang W et al. The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development. Nat Commun 13:958 (2022). PubMed: 35177643
- Zhao YN et al. Whole-Brain Monosynaptic Afferents to Rostromedial Tegmental Nucleus Gamma-Aminobutyric Acid-Releasing Neurons in Mice. Front Neurosci 16:914300 (2022). PubMed: 35733933
- Li Z et al. Transcription factor Sp9 is a negative regulator of D1-type MSN development. Cell Death Discov 8:301 (2022). PubMed: 35773249