Anti-FOXO4 (phospho S197)抗体(ab47278)

概述

  • 产品名称Anti-FOXO4 (phospho S197)抗体
    参阅全部 FOXO4 一抗
  • 描述
    兔多克隆抗体to FOXO4 (phospho S197)
  • 特异性This (phospho-Ser197) antibody detects endogenous levels of FOXO4 only when phosphorylated at serine 197.
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized phosphopeptide derived from human FOXO4 around the phosphorylation site of serine 197 (A-A-SP-M-D)

  • 阳性对照
    • Human breast carcinoma tissue, 293 cell extract.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
IHC-P
ELISA
  • 应用说明ELISA: 1/20000.
    IHC: 1/50 - 1/100.
    ICC/IF: Use at a concentration of 1-5 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 54 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
    • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
    • 疾病相关Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
    • 序列相似性Contains 1 fork-head DNA-binding domain.
    • 翻译后修饰Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
    • 细胞定位Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AFX antibody
      • AFX1 antibody
      • Afxh antibody
      • ALL1-fused gene from X chromosome antibody
      • Fork head domain transcription factor AFX1 antibody
      • Forkhead box O4 antibody
      • Forkhead box protein O4 antibody
      • FOXO 4 antibody
      • Foxo4 antibody
      • FOXO4_HUMAN antibody
      • MGC117660 antibody
      • MGC120490 antibody
      • Mixed lineage leukemia, translocated to, 7 antibody
      • MLLT7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
      • RGD1561201 antibody
      see all

    Anti-FOXO4 (phospho S197) antibody 图像

    • Immunohistochemical analysis of paraffinembedded human breast carcinoma tissue using ab47278. Right : peptide 1ug/ml
    • All lanes : Anti-FOXO4 (phospho S197) antibody (ab47278) at 1/500 dilution

      Lane 1 : Extracts from 293 cells.
      Lane 2 : Extracts from 293 cells. Immunizing peptide 1ug/mL

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 54 kDa
    • ICC/IF image of ab47278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-FOXO4 (phospho S197) antibody (ab47278)参考文献

    ab47278 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (liver)
    Loading amount 70 µg
    Specification liver
    Treatment serum starvation
    Gel Running Conditions Non-reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Jun 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"