概述

  • 产品名称Anti-FOXO4抗体
    参阅全部 FOXO4 一抗
  • 描述
    兔多克隆抗体to FOXO4
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human FOXO4 around the phosphorylation site of threonine 451

  • 阳性对照
    • K562 cells, HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab63254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • 疾病相关Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
  • 细胞定位Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • RGD1561201 antibody
    see all

Anti-FOXO4 antibody 图像

  • All lanes : Anti-FOXO4 antibody (ab63254) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells, with immunizing peptide


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Immunofluorescence analysis of FOXO4 expression in HeLa cells, using 1/500 ab63254. Left: untreated sample. Right: sample treated with immunising peptide.
  • Ab63254 staining Human normal pancreas. Staining is localised to the nucleus and cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-FOXO4 antibody (ab63254)参考文献

This product has been referenced in:
  • Liu X  et al. MicroRNA-499-5p promotes cellular invasion and tumor metastasis in colorectal cancer by targeting FOXO4 and PDCD4. Carcinogenesis 32:1798-805 (2011). WB ; Human . Read more (PubMed: 21934092) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step Other
Sample Mouse Tissue sections (chondrocytes)
Specification chondrocytes
Permeabilization No
Fixative zinc buffered formalin
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Verified customer

提交于 Mar 17 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (fibroblast)
Specification fibroblast
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"