概述

  • 产品名称Anti-FOXO4抗体
    参阅全部 FOXO4 一抗
  • 描述
    兔多克隆抗体to FOXO4
  • 特异性This antibody is specific for FOXO4.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized non-phosphopeptide derived from human FOXO4 around the phosphorylation site of serine 197.

  • 阳性对照
    • Extracts from 293 cells and human lung carcinoma tissue

性能

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应用

Our Abpromise guarantee covers the use of ab47396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • 疾病相关Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
  • 细胞定位Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • RGD1561201 antibody
    see all

Anti-FOXO4 antibody 图像

  • All lanes : Anti-FOXO4 antibody (ab47396) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with serum
    Lane 2 : Extracts from 293 cells treated with serum and peptide


    Predicted band size : 54 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab47396 at a 1/50 dilution.

    Left image : Un-treated

    Right image : Treated with peptide

Anti-FOXO4 antibody (ab47396)参考文献

This product has been referenced in:
  • Iwata J  et al. Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells. J Biol Chem 285:4975-82 (2010). WB ; Mouse . Read more (PubMed: 19959467) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T transfected with epitope-tagged FOXO4)
Loading amount 20 µg
Specification 293T transfected with epitope-tagged FOXO4
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jul 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"