Anti-FOXO3A抗体(ab70315)
Key features and details
- Rabbit polyclonal to FOXO3A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-FOXO3A抗体
参阅全部 FOXO3A 一抗 -
描述
兔多克隆抗体to FOXO3A -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 625 and the C terminus (residue 673) of human FOXO3A. (Swiss-Prot O43524).
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阳性对照
- Whole cell lysate from MCF7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70315于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
细胞定位
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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数据库链接
- Entrez Gene: 535530 Cow
- Entrez Gene: 481954 Dog
- Entrez Gene: 100066558 Horse
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 733621 Pig
- Entrez Gene: 294515 Rat
- Omim: 602681 Human
see all -
别名
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
图片
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All lanes : Anti-FOXO3A antibody (ab70315) at 0.1 µg/ml
Lane 1 : HeLa cell Lysate
Lane 2 : 293T cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : Mouse TCMK-1 cell Lysate
Lane 5 : Mouse NIH3T3 cell Lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes -
Lane 1: MCF7 cell Lysate immunoprecipitated with ab70314 at 6 ug/mg of Lysate
Lane 2: MCF7 cell Lysate immunoprecipitated with ab70315 at 6 ug/mg of Lysate
Lane 3: MCF7 cell Lysate immunoprecipitated with Control IgG at 6 ug/mg of Lysate
Western Blot carried out with ab70315 at 1ug/ml.
Dectection by ECL for 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70315 at 1/1000 (1µg/ml). Detection: DAB.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab70315 被引用在 11 文献中.
- Zhu X et al. Elaiophylin Inhibits Tumorigenesis of Human Uveal Melanoma by Suppressing Mitophagy and Inducing Oxidative Stress via Modulating SIRT1/FoxO3a Signaling. Front Oncol 12:788496 (2022). PubMed: 35387119
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- Zhang L et al. Role and mechanism underlying FoxO6 in skeletal muscle in vitro and in vivo. Int J Mol Med 48:N/A (2021). PubMed: 34080654
- Cao Y et al. SIRT3 promotion reduces resistance to cisplatin in lung cancer by modulating the FOXO3/CDT1 axis. Cancer Med 10:1394-1404 (2021). PubMed: 33655712
- Zhao N et al. Androgen Receptor, Although Not a Specific Marker For, Is a Novel Target to Suppress Glioma Stem Cells as a Therapeutic Strategy for Glioblastoma. Front Oncol 11:616625 (2021). PubMed: 34094902