概述
-
产品名称Anti-FOXO3A抗体
参阅全部 FOXO3A 一抗 -
描述兔多克隆抗体to FOXO3A
-
宿主Rabbit
-
经测试应用适用于: WB, IP, IHC-Pmore details
-
种属反应性与反应: Mouse, Human
预测可用于: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
-
免疫原
Synthetic peptide corresponding to a region between residues 250 and 300 of human FOXO3A. (Swiss-Prot O43524)
-
阳性对照
- Whole cell lysate from MCF7 cells
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
存储溶液Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
-
Positive Controls
应用
Our Abpromise guarantee covers the use of ab70314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000 - 1/15000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
-
功能Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
-
组织特异性Ubiquitous.
-
疾病相关Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
-
序列相似性Contains 1 fork-head DNA-binding domain.
-
翻译后修饰In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
-
细胞定位Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
- Information by UniProt
-
数据库链接
- Entrez Gene: 535530 Cow
- Entrez Gene: 481954 Dog
- Entrez Gene: 100066558 Horse
- Entrez Gene: 2309 Human
- Entrez Gene: 56484 Mouse
- Entrez Gene: 733621 Pig
- Omim: 602681 Human
- SwissProt: O43524 Human
see all -
别名
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
图片
-
Immunoprecipitation - Anti-FOXO3A antibody (ab70314)Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg.
Lane 2: MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3:MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Western Blot carried out using ab70314 at 1ug/ml
Decction: ECL for 3 minutes.
All lanes : Anti-FOXO3A antibody (ab70314) at 1 µg/ml
Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg
Lane 2 : MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3 : MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Developed using the ECL technique.
Exposure time: 3 minutes -
Western blot - Anti-FOXO3A antibody (ab70314)All lanes : Anti-FOXO3A antibody (ab70314) at 0.4 µg/ml
Lane 1 : 293T cell Lysate
Lane 2 : Jurkat cell Lysate
Lane 3 : Mouse TCMK cell Lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab70314)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70314 at 1/500 (2µg/ml). Detection: DAB.
文献
ab70314 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"