概述

性能

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应用

Our Abpromise guarantee covers the use of ab17026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 72 kDa).Can be blocked with Human FOXO3A peptide (ab45581).
ELISA 1/32000.

靶标

  • 功能Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
  • 组织特异性Ubiquitous.
  • 疾病相关Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AF6q21 antibody
    • AF6q21 protein antibody
    • DKFZp781A0677 antibody
    • FKHR2 antibody
    • FKHRL 1 antibody
    • FKHRL1 antibody
    • FKHRL1P2 antibody
    • Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
    • Forkhead box O3 antibody
    • Forkhead box O3A antibody
    • Forkhead box protein O3 antibody
    • Forkhead box protein O3A antibody
    • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
    • Forkhead homolog (rhabdomyosarcoma) like 1 antibody
    • Forkhead in rhabdomyosarcoma like 1 antibody
    • Forkhead in rhabdomyosarcoma-like 1 antibody
    • FOX O3A antibody
    • FOXO2 antibody
    • foxo3 antibody
    • FOXO3_HUMAN antibody
    • FOXO3A antibody
    • MGC12739 antibody
    • MGC31925 antibody
    see all

Anti-FOXO3A antibody 图像

  • Anti-FOXO3A antibody (ab17026) at 0.1 µg/ml + Human brain lysates at 35 µg

    Predicted band size : 72 kDa
    ab17026 at 0.5ug/ml on human brain lysate (35ug per lane).
  • All lanes : Anti-FOXO3A antibody (ab17026) at 1/1000 dilution

    Lane 1 : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate
    Lane 2 : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate
    Lane 3 : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase conjugated-mouse anti-goat IgG monoclonal at 1/15000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    Blocked for 1 hour at 22°C.

    Incubated with the primary antibody for 20 hours at 4°C.

    See Abreview

Anti-FOXO3A antibody (ab17026)参考文献

This product has been referenced in:
  • Stefanetti RJ  et al. Ageing has no effect on the regulation of the ubiquitin proteasome-related genes and proteins following resistance exercise. Front Physiol 5:30 (2014). WB ; Human . Read more (PubMed: 24550841) »
  • Raeis V  et al. Central venous hypoxemia is a determinant of human atrial ATP-sensitive potassium channel expression: evidence for a novel hypoxia-inducible factor 1alpha-Forkhead box class O signaling pathway. Hypertension 55:1186-92 (2010). Read more (PubMed: 20212266) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (human hepatic epithelial-like cell line (AKN-1))
Gel Running Conditions Non-reduced Denaturing (8%)
Loading amount 30 µg
Specification human hepatic epithelial-like cell line (AKN-1)
Blocking step I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 14 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 100000 cells
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCap (prostate cancer cell line))
Loading amount 10 µg
Specification LNCap (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (7% tris-acetate)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2008

Thank you for your enquiry and patience. The originator of ab17026, who also characterized the antibody, was able to provide me with the following information regarding their protocol. I hope this is helpful and if you need additional assistance, pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"