Anti-FOXO3A抗体(ab17026)
Key features and details
- Goat polyclonal to FOXO3A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FOXO3A抗体
参阅全部 FOXO3A 一抗 -
描述
山羊多克隆抗体to FOXO3A -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
GAKQASSQSWVPG
, corresponding to amino acids 661-673 of Human FOXO3A. -
阳性对照
- Recombinant Human FOXO3A protein (ab114191) can be used as a positive control in WB. Human heart tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17026于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
序列相似性
Contains 1 fork-head DNA-binding domain. -
翻译后修饰
In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
细胞定位
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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数据库链接
- Entrez Gene: 2309 Human
- Omim: 602681 Human
- SwissProt: O43524 Human
- Unigene: 220950 Human
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别名
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
图片
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All lanes : Anti-FOXO3A antibody (ab17026) at 0.3 µg/ml
Lane 1 : Human Heart tissue lysate
Lane 2 : Human Heart tissue lysate with immunising peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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All lanes : Anti-FOXO3A antibody (ab17026) at 1/1000 dilution
All lanes : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase conjugated-mouse anti-goat IgG monoclonal at 1/15000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 72 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible non-specific binding)
Exposure time: 5 minutesBlocked for 1 hour at 22°C.
Incubated with the primary antibody for 20 hours at 4°C.
数据表及文件
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Datasheet download
文献 (17)
ab17026 被引用在 17 文献中.
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Yeh CC et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep 25:N/A (2022). PubMed: 34913071
- Du Y & Ning JZ MiR-182 Promotes Ischemia/Reperfusion-Induced Acute Kidney Injury in Rat by Targeting FoxO3. Urol Int 105:687-696 (2021). PubMed: 33965964
- Li Z et al. Kcnh2 mediates FAK/AKT-FOXO3A pathway to attenuate sepsis-induced cardiac dysfunction. Cell Prolif 54:e12962 (2021). PubMed: 33263944
- Lu X et al. Modulation of HOXA9 after skeletal muscle denervation and reinnervation. Am J Physiol Cell Physiol 318:C1154-C1165 (2020). PubMed: 32233950