Anti-FOXO1A (phospho S319)抗体(ab47326)


  • 产品名称
    Anti-FOXO1A (phospho S319)抗体
    参阅全部 FOXO1A 一抗
  • 描述
    兔多克隆抗体to FOXO1A (phospho S319)
  • 宿主
  • 特异性
    This antibody detects endogenous levels of FOXO1A only when phosphorylated at serine 319.(Human: Ser319; Mouse: Ser316; Rat: Ser313)
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319 (T-S-SP-N-A)

  • 阳性对照
    • human breast carcinoma, HeLa cells.



Our Abpromise guarantee covers the use of ab47326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明
    ELISA: 1/10000.
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
    • 组织特异性
    • 疾病相关
      Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
    • 序列相似性
      Contains 1 fork-head DNA-binding domain.
    • 翻译后修饰
      Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FKH 1 antibody
      • FKH1 antibody
      • FKHR antibody
      • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
      • Forkhead box O1 antibody
      • Forkhead box protein O1 antibody
      • Forkhead box protein O1A antibody
      • Forkhead in rhabdomyosarcoma antibody
      • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
      • FoxO transcription factor antibody
      • foxo1 antibody
      • FOXO1_HUMAN antibody
      • FOXO1A antibody
      • OTTHUMP00000018301 antibody
      see all


    • ab47326 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
    • All lanes : Anti-FOXO1A (phospho S319) antibody (ab47326)

      Lane 1 : extracts from EGF treated HeLa cells
      Lane 2 : extracts from HeLa cells

      Predicted band size: 70 kDa

      Typically 5-30ug of total protein was loaded per lane of the gel.
    • ICC/IF image of ab47326 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


    ab47326 has not yet been referenced specifically in any publications.


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