概述

  • 产品名称Anti-FOXO1A抗体
    参阅全部 FOXO1A 一抗
  • 描述
    兔多克隆抗体to FOXO1A
  • 经测试应用适用于: ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken, Pig
  • 免疫原

    Synthetic peptide:

    LPNQSFPHSVKTTTHS

    conjugated to KLH, corresponding to amino acids 636-651 of Human FOXO1A.

  • 阳性对照
    • ICC: mouse E11 cortical cells cultured for 6 days WB: HEK293 cell lysate, HeLa cell lysate
  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

性能

应用

Our Abpromise guarantee covers the use of ab12161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
ICC 1/200 - 1/1000.
WB 1/2500. Detects a band of approximately 70-75 kDa (predicted molecular weight: 70 kDa).

靶标

  • 功能Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Anti-FOXO1A antibody 图像

  • ab12161 staining FOXO1A (1/200) in mouse E11 cortical cells cultured for 6 days. Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.
  • ab12161 staining FOXOA1 (1/200) in the cortical plate (CP) and ventricular zone (VZ=CNS germinal zone) of mouse E13 forebrain tissue. E13 mouse embryos were fixed in 4% paraformaldehyde in PB overnight at 4°C then transferred to 30% sucrose in PBS.  Brains were frozen in TissueTek and stored at -80°C. Cryostat coronal sections were cut at 12µm. Sections were blocked in 0.1% triton in PBS containing 10% normal serum. Primary antibodies were incubated on the sections at 4°C overnight.  Secondary antibody Alexa 546 goat anti rabbit IgG 1/1000 was incubated for 2 hours at room temperature. Images were taken using a Zeiss camera under 40X objectives.
  • All lanes : Anti-FOXO1A antibody (ab12161) at 1 µg/ml

    Lane 1 : HEK293 whole cell lysate (ab7902)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa (possible cross reactivity).

Anti-FOXO1A antibody (ab12161)参考文献

This product has been referenced in:
  • Picard M  et al. Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogramming. Proc Natl Acad Sci U S A 111:E4033-42 (2014). WB . Read more (PubMed: 25192935) »
  • Awad H  et al. AMPK and FoxO1 regulate catalase expression in hypoxic pulmonary arterial smooth muscle. Pediatr Pulmonol 49:885-97 (2014). Read more (PubMed: 24167160) »

See all 7 Publications for this product

Product Wall

Thank you for your email. I have instructed our accounting department to issue you a refund for your order for ab12161. As the order was placed using a purchase order number, this process does take a little while. If you have any questions regarding th...

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Thank you for your email. I can send you a free of charge replacement vial of ab12161, or I can issue you a credit note or refund. Please let me know which you would prefer. Thank you, and I look forward to hearing from you.

Thank you for your email and the image that you sent. There is a definite improvement with regards to background and non-specific banding. Did you filter the TBST buffer that you are using? Failure to filter can lead to “spotting” where tiny dark g...

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Thank you for your email and for sending images of your blots. I can definitely see that you are experiencing difficulty with high background and non-specific bands. With this antibody you should see a band at approximately 70-75 kDa. It would be h...

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Thank you for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets. We currently do not have information about specificity to O1A/o1a and other FOXO/foxo family members. If you have any more ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"