概述

  • 产品名称Anti-FOXM1抗体
    参阅全部 FOXM1 一抗
  • 描述
    兔多克隆抗体to FOXM1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 714-763 (ANRSLTEGLV LDTMNDSLSK ILLDISFPGL DEDPLGPDNI NWSQFIPELQ) of human FOXM1 (NP_068772).

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Transcriptional activatory factor. May play a role in the control of cell proliferation.
  • 组织特异性Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 发展阶段Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus.
  • 结构域Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.
  • 翻译后修饰Phosphorylated in M (mitotic) phase.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FKHL16 antibody
    • Forkhead box M1 antibody
    • Forkhead box protein M1 antibody
    • forkhead like 16 antibody
    • Forkhead-related protein FKHL16 antibody
    • FOX M1 antibody
    • Foxm1 antibody
    • FOXM1_HUMAN antibody
    • FOXM1B antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
    • HFH-11 antibody
    • HFH11 antibody
    • HNF-3/fork-head homolog 11 antibody
    • HNF3 antibody
    • INS1 antibody
    • M phase phosphoprotein 2 antibody
    • M-phase phosphoprotein 2 antibody
    • MPHOSPH2 antibody
    • MPM-2 reactive phosphoprotein 2 antibody
    • MPP2 antibody
    • PIG29 antibody
    • TGT3 antibody
    • Transcription factor Trident antibody
    • Trident antibody
    • WIN antibody
    • Winged-helix factor from INS-1 cells antibody
    • Winged-helix factor from INS1 cells antibody
    see all

Anti-FOXM1 antibody 图像

  • ab83097 at 5 µg/ml staining FOXM1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-FOXM1 antibody (ab83097) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Gel concentration 12%

Anti-FOXM1 antibody (ab83097)参考文献

This product has been referenced in:
  • Hu C  et al. LXRa-mediated downregulation of FOXM1 suppresses the proliferation of hepatocellular carcinoma cells. Oncogene N/A:N/A (2013). Read more (PubMed: 23812424) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas (Islets))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Declere
Permeabilization No
Specification Pancreas (Islets)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
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提交于 Apr 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"