概述

性能

应用

Our Abpromise guarantee covers the use of ab5096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45-50 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • 组织特异性In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
  • 疾病相关Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) [MIM:110100]; also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II.
    Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3) [MIM:608996]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Blepharophimosis antibody
    • Blepharophimosis epicanthus inversus and ptosis 1 antibody
    • Blepharophimosis epicanthus inversus and ptosis antibody
    • BPES 1 antibody
    • BPES antibody
    • BPES1 antibody
    • Epicanthus inversus and ptosis 1 antibody
    • Forkhead box L2 antibody
    • Forkhead box protein L2 antibody
    • Forkhead transcription factor FOXL2 antibody
    • FOX L2 antibody
    • FOXL 2 antibody
    • FOXL2 antibody
    • FOXL2_HUMAN antibody
    • PFRK antibody
    • PINTO antibody
    • PITUITARY FORKHEAD FACTOR antibody
    • POF 3 antibody
    • POF3 antibody
    see all

Anti-FOXL2 antibody 图像

  • All lanes : Anti-FOXL2 antibody (ab5096) at 1 µg/ml

    Lane 1 : Human ovary lysate (RIPA Buffer)
    Lane 2 : Mouse ovary lysate (RIPA Buffer)

    Lysates/proteins at 35 µg/ml per lane.


    Predicted band size : 43 kDa
  • Immunohistochemical analysis of paraffin embedded Human Ovary tissue labeling FOXL2 with ab5096 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.

  • Anti-FOXL2 antibody (ab5096) at 0.5 µg/ml + Human heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Anti-FOXL2 antibody (ab5096) at 0.5 µg/ml + Human Ovary lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 43 kDa

    Primary incubation was for 1 hour.

  • ab5096 staining FOXL2 in female mice oocytes by Immunohistochemistry (PFA-fixed paraffin-embedded sections).
    Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody ab5096 at a 1/100, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

    Ovarian cord structure was examined by TRA98 germ cell and FOXL2 granulosa somatic cell immunofluorescence over 12 days of e12.5 female genital ridge transplantation. Oocytes and granulosa cells clustered together in defi

Anti-FOXL2 antibody (ab5096)参考文献

This product has been referenced in:
  • Governini L  et al. FOXL2 in Human Endometrium: Hyperexpressed in Endometriosis. Reprod Sci N/A:N/A (2014). IHC ; Human . Read more (PubMed: 24520083) »
  • Georg I  et al. Sox9 and Sox8 Are Required for Basal Lamina Integrity of Testis Cords and for Suppression of FOXL2 During Embryonic Testis Development in Mice. Biol Reprod 87:99 (2012). Mouse . Read more (PubMed: 22837482) »

See all 10 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (ovary)
Permeabilization No
Specification ovary
Blocking step 10% donkey serum, 0.2% Triton x100, PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Prof. SK Roy

Verified customer

提交于 Mar 31 2016

Application Western blot
Sample Rat Tissue lysate - whole (Gonad)
Gel Running Conditions Reduced Denaturing (10% SDA-PAGE)
Loading amount 75 µg
Specification Gonad
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Ovary)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Ovary
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 14 2015

Merci pour votre réponse.

J'ai comme convenu mis en place l'envoi d'une unité de ab5096. Le numéro de commande de remplacement gratuit de ce produit est *****.
Vous recevrez prochainement un mail de confirmation compre...

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A ma connaissance, le test en ICC/IF des anticorps cités dans votre email n'est malheureusement pas prévu.

Personnellement j'essaierais à nouveau le ab5096. Si le problème persiste je conseillerai dans ce cas de tester le ab74144, spécifique d...

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Merci pour votre réponse.

Malheureusement il ne nous est pas possible de fournir des échantillons de chaque référence car nous avons plus de 80.000 références anticorps et pour des raisons logistiques ...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le dernier tube de ab5096 que vous avez reçu ne fonctionne pas comme attendu.

Afin de mieux comprendre le problème et vous apporter ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (DU145 (prostate cancer cell line)
Loading amount 10 µg
Specification DU145 (prostate cancer cell line
Gel Running Conditions Reduced Denaturing (7% tris-acetate)
Blocking step Milk as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 24 2008

Thank you for your enquiry. I am sorry but we do not currently have the synthetic immunogen peptide used to raise FOXL2 antibody (ab5096). The immunogen was a peptide of sequence DSKTGALHSRLDL. I am not aware of any modifications that were used in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"