Anti-FOXJ2抗体(ab22857)

概述

  • 产品名称Anti-FOXJ2抗体
    参阅全部 FOXJ2 一抗
  • 描述
    兔多克隆抗体to FOXJ2
  • 特异性ab22857 reacts with Forkhead box protein J2 (FOXJ2).
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    A region within synthetic peptide: AGASGRESAE GPPPLYNTNH DFKFSYSEIN FQDLSWSFRN LYKSMLEKSS, corresponding to internal sequence amino acids 218-267 of Human FOXJ2

  • 阳性对照
    • HepG2 cell lysate and human stomach

性能

应用

Our Abpromise guarantee covers the use of ab22857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FHX antibody
    • Fork head homologous X antibody
    • Forkhead box J2 antibody
    • Forkhead box protein J2 antibody
    • FOXJ2 antibody
    • FOXJ2_HUMAN antibody
    see all

Anti-FOXJ2 antibody 图像

  • Anti-FOXJ2 antibody (ab22857) at 2 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab22857, at 4 µg/ml, staining human FOXJ2 in human Stomach by Immunohistochemistry, Paraffin embedded tissue. Positive stained cells, indicated with arrows, are epithelial cells of fundic gland. Magnification 400 x.

Anti-FOXJ2 antibody (ab22857)参考文献

ab22857 has not yet been referenced specifically in any publications.

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