Anti-FOXJ2抗体(ab22857)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FOXJ2
  • 特异性
    ab22857 reacts with Forkhead box protein J2 (FOXJ2).
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    A region within synthetic peptide: AGASGRESAE GPPPLYNTNH DFKFSYSEIN FQDLSWSFRN LYKSMLEKSS, corresponding to internal sequence amino acids 218-267 of Human FOXJ2

  • 阳性对照
    • HepG2 cell lysate and human stomach

性能

应用

Our Abpromise guarantee covers the use of ab22857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FHX antibody
    • Fork head homologous X antibody
    • Forkhead box J2 antibody
    • Forkhead box protein J2 antibody
    • FOXJ2 antibody
    • FOXJ2_HUMAN antibody
    see all

图片

  • Anti-FOXJ2 antibody (ab22857) at 2 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab22857, at 4 µg/ml, staining human FOXJ2 in human Stomach by Immunohistochemistry, Paraffin embedded tissue. Positive stained cells, indicated with arrows, are epithelial cells of fundic gland. Magnification 400 x.

文献

ab22857 has not yet been referenced specifically in any publications.

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