Anti-FOXG1抗体- ChIP Grade (ab18259)

概述

  • 产品名称
    Anti-FOXG1抗体- ChIP Grade
    参阅全部 FOXG1 一抗
  • 描述
    兔多克隆抗体to FOXG1 - ChIP Grade
  • 经测试应用
    适用于: WB, ChIP, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human FOXG1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P55316
    (Peptide available as ab19644)

  • 阳性对照
    • This antibody gave a positive signal in Human, Mouse and Rat brain tissue lysates. IHC-P: FFPE mouse brain E14.

性能

应用

Our Abpromise guarantee covers the use of ab18259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ChIP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/1000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
  • 组织特异性
    Expression is restricted to the neurons of the developing telencephalon.
  • 疾病相关
    Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.
  • 序列相似性
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BF-1 antibody
    • BF-2 antibody
    • BF1 antibody
    • BF2 antibody
    • Brain factor 1 antibody
    • Brain factor 2 antibody
    • FHKL antibody
    • FKH2 antibody
    • FKHL1 antibody
    • FKHL2 antibody
    • FKHL3 antibody
    • FKHL4 antibody
    • Forkhead box protein G1 antibody
    • Forkhead box protein G1A antibody
    • Forkhead box protein G1B antibody
    • Forkhead box protein G1C antibody
    • Forkhead like 1 antibody
    • Forkhead like 2 antibody
    • Forkhead like 3 antibody
    • Forkhead like 4 antibody
    • Forkhead-related protein FKHL1 antibody
    • Forkhead-related protein FKHL2 antibody
    • Forkhead-related protein FKHL3 antibody
    • FOXG1 antibody
    • FOXG1_HUMAN antibody
    • FOXG1A antibody
    • FOXG1B antibody
    • FOXG1C antibody
    • HBF 1 antibody
    • HBF G2 antibody
    • hBF-2 antibody
    • HBF2 antibody
    • HFK1 antibody
    • HFK2 antibody
    • HFK3 antibody
    • KHL2 antibody
    • Oncogene QIN antibody
    • QIN antibody
    see all

Anti-FOXG1 antibody - ChIP Grade 图像

  • IHC image of FoxG1 staining in mouse brain E14 formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18259, 0.5 µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Staining revealed in telencephalon (but not diencephalon) as expected.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab18259 staining FOXG1 in Rat embryonic cortex cells overexpressing FOXG1 by ICC/IF (Immunocytochemistry/ immunofluorescence). Cells were fixed with formaldehyde and blocked with 1o% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 24 hours at 4°C. A Cy3®-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • All lanes : Anti-FOXG1 antibody - ChIP Grade (ab18259) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Human spleen tissue lysate - total protein (negative control)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 155 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-FOXG1 antibody - ChIP Grade (ab18259) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human FOXG1 peptide (ab19644) at 1 µg/ml
    Lane 5 : Brain (Mouse) Tissue Lysate with Human FOXG1 peptide (ab19644) at 1 µg/ml
    Lane 6 : Brain (Rat) Tissue Lysate with Human FOXG1 peptide (ab19644) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 1 minute

    ab18259 detects a band of ~50kDa in brain lysates. This band is blocked by addition of the immunizing peptide ab19644 which suggests that is specific for FOXG1.

     

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution.

  • ab18259 staining Foxg1 (1/50) in the telencephalon (but not the diencephalon) as expected. DAB-immunohistochemistry was performed on embryonic (E13.5) mouse brain (coronal paraffin-embedded sections) after microwave treatment with 10mM sodium citrate.

  • ab18259 staining FOXG1 in human A549 cells by immunocytochemistry/immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab18259 at a 1/400 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat polyclonal used at a 1/250 dilution.

    See Abreview

Anti-FOXG1 antibody - ChIP Grade (ab18259)参考文献

This product has been referenced in:
  • Subramanian L  et al. Dynamic behaviour of human neuroepithelial cells in the developing forebrain. Nat Commun 8:14167 (2017). IF ; Human . Read more (PubMed: 28139695) »
  • Xu CC  et al. Abnormal mitochondrial transport and morphology as early pathological changes in human models of spinal muscular atrophy. Dis Model Mech 9:39-49 (2016). Human . Read more (PubMed: 26586529) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human cerebral organoids)
Specification
Human cerebral organoids
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Formaldehyde
Username

Radhika Menon

Verified customer

提交于 Sep 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain Tissue)
Permeabilization
Yes - 0.01% triton
Specification
Brain Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Yasir Ahmed Syed

Verified customer

提交于 Jun 16 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cortical neurons)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
cortical neurons
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 07 2016

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (undifferentiated human iPS cells)
Specification
undifferentiated human iPS cells
Blocking step
5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 24 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample
Human Cell (human neural progenitor cell from ips cell line)
Specification
human neural progenitor cell from ips cell line
Permeabilization
Yes - see blocking buffer
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 24 2014

After reviewing your protocol, I have some recommendations that are likely to improve your results:

1. Some epitopes are sensitive to methanol fixation, and both of the researchers in the abReviews below saw good results using a 3-4% paraforma...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (FOXG1 overexpressed rat embryonic cortex)
Specification
FOXG1 overexpressed rat embryonic cortex
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 10 2013

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human fibroblast and stem cell)
Loading amount
50 µg
Specification
human fibroblast and stem cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2012

Thank you for submitting an Abreview of ab18259. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

Read More

1-10 of 25 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册