概述

  • 产品名称
  • 描述
    兔多克隆抗体to FOXD3
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthesized peptide derived from an internal region of human FOXD3.

  • 阳性对照
    • Human heart tissue and extracts from HUVEC and 293 cells

性能

应用

Our Abpromise guarantee covers the use of ab64807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ELISA 1/10000.

靶标

  • 功能
    Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
  • 组织特异性
    Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
  • 疾病相关
    Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis).
  • 序列相似性
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIS1 antibody
    • forkhead box D3 antibody
    • Forkhead box protein D3 antibody
    • foxd3 antibody
    • FOXD3_HUMAN antibody
    • Genesis antibody
    • HFH2 antibody
    • HNF3/FH transcription factor genesis antibody
    • VAMAS2 antibody
    see all

图片

  • All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution

    Lane 1 : Extracts of HUVEC cells
    Lane 2 : Extracts of 293 cells
    Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
    Left image untreated.
    Right image treated with immunising peptide.

文献

This product has been referenced in:
  • Douglas GC  et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). Read more (PubMed: 19299251) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your inquiry.

I contacted the producing labs of these two antibodies. Although the immunogen sequences are confidential, they did an alignment with the sequence you provided.

Unfortunately, both immunogen sequences did...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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