Anti-FOXD3抗体(ab64807)
Key features and details
- Rabbit polyclonal to FOXD3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FOXD3抗体
参阅全部 FOXD3 一抗 -
描述
兔多克隆抗体to FOXD3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
A synthesized peptide derived from an internal region of human FOXD3.
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阳性对照
- Human heart tissue and extracts from HUVEC and 293 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64807于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. -
组织特异性
Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined. -
疾病相关
Defects in FOXD3 are associated with susceptibility to autoimmune disease type 1 (AIS1) [MIM:607836]. AIS1 is a disorder characterized by the association of vitiligo with autoimmune thyroiditis (Hashimoto thyroiditis). -
序列相似性
Contains 1 fork-head DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 27022 Human
- Entrez Gene: 15221 Mouse
- Omim: 611539 Human
- SwissProt: Q9UJU5 Human
- SwissProt: Q61060 Mouse
- Unigene: 546573 Human
- Unigene: 4758 Mouse
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别名
- AIS1 antibody
- forkhead box D3 antibody
- Forkhead box protein D3 antibody
see all
图片
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All lanes : Anti-FOXD3 antibody (ab64807) at 1/500 dilution
Lane 1 : Extracts of HUVEC cells
Lane 2 : Extracts of 293 cells
Lane 3 : Extracts of 293 cells, plus immunising peptide at 5-10µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64807 at a 1:50 dilution.
Left image untreated.Right image treated with immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab64807 被引用在 4 文献中.
- Liu R et al. FOXD3 and GAB2 as a pair of rivals antagonistically control hepatocellular carcinogenesis. FEBS J 289:4536-4548 (2022). PubMed: 35178865
- Cheng Y et al. FOXD3-induced miR-133a blocks progression and metastasis of colorectal cancer through regulating UBA2. J Cancer 12:6145-6154 (2021). PubMed: 34539887
- Huang J & Chen L IL-1β inhibits osteogenesis of human bone marrow-derived mesenchymal stem cells by activating FoxD3/microRNA-496 to repress wnt signaling. Genesis 55:N/A (2017). PubMed: 28509407
- Douglas GC et al. Trophoblast stem cells: models for investigating trophectoderm differentiation and placental development. Endocr Rev 30:228-40 (2009). PubMed: 19299251