概述

  • 产品名称Anti-FOXD2抗体
    参阅全部 FOXD2 一抗
  • 描述
    兔多克隆抗体to FOXD2
  • 特异性This antibody recognizes FOXD2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide: GELPARSGPR APRDVLPHGH EPPAEEAEAD LAEDEEESGG CSDGEPRALA, corresponding to a region within N terminal amino acids 37-86 of Human FOXD2

  • 阳性对照
    • Jurkat cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab49128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:62500.

WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-FOXD2 antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-FOXD2 antibody (ab49128) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-FOXD2 antibody (ab49128)参考文献

ab49128 has not yet been referenced specifically in any publications.

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