This antibody recognizes FOXD2
Synthetic peptide: GELPARSGPR APRDVLPHGH EPPAEEAEAD LAEDEEESGG CSDGEPRALA, corresponding to a region within N terminal amino acids 37-86 of Human FOXD2
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay was 1:62500.
||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Probable transcription factor involved in embryogenesis and somatogenesis.
Contains 1 fork-head DNA-binding domain.
Information by UniProt
- Drosphilia Forkhead Homolog Like 17 antibody
- FKHL 17 antibody
- Forkhead box protein D2 antibody
Western blot - FOXD2 antibody (ab49128)
Lane 1 :
MarkerLane 2 :
Anti-FOXD2 antibody (ab49128) at 1.25 µg/mlLane 1 :
As aboveLane 2 :
Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.SecondaryLane 2 :
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
49 kDaObserved band size :
50 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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