Anti-FOXC2抗体- ChIP Grade (ab5060)

概述

  • 产品名称Anti-FOXC2抗体- ChIP Grade
    参阅全部 FOXC2 一抗
  • 描述
    山羊多克隆抗体to FOXC2 - ChIP Grade
  • 特异性The antibody labels FOXC2 in mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries. In western blotting the antibody has been tested on FOXC2 overexpressing cell lines but not on endogenous protein.
  • 经测试应用适用于: WB, ChIP, IHC-Pmore details
    不适用于: IHC-Fr
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    RHAAPYSYDCTKY

    , corresponding to C terminal amino acids 489-501 of Human FOXC2.

  • 阳性对照
    • IHC: mouse lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries WB: FOXC2 overexpressing cell lines

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab5060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human FOXC2 peptide (ab23061).
ChIP Use at an assay dependent concentration. PubMed: 18579532
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for IHC-Fr.
  • 靶标

    • 功能Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.
    • 疾病相关Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200]; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.
      Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300]. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities.
      Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400]. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).
    • 序列相似性Contains 1 fork-head DNA-binding domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Drosphilia Forkhead Homolog Like 14 antibody
      • FKHL 14 antibody
      • FKHL14 antibody
      • Forkhead Box C2 antibody
      • Forkhead box protein C2 antibody
      • Forkhead related protein FKHL14 antibody
      • Forkhead-related protein FKHL14 antibody
      • FOX C2 antibody
      • Foxc2 antibody
      • FOXC2_HUMAN antibody
      • LD antibody
      • Mesenchyme fork head protein 1 antibody
      • Mesenchyme Forkhead 1 antibody
      • MFH 1 antibody
      • MFH 1 protein antibody
      • MFH-1 protein antibody
      • MFH1 antibody
      • Transcription factor FKH 14 antibody
      • Transcription factor FKH-14 antibody
      see all

    Anti-FOXC2 antibody - ChIP Grade 图像

    • ab5060 (2µg/ml) staining of paraffin embedded Human Breast cancer tissue shows nuclear staning with some weak cytoplasm staining. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.



    • Predicted band size : 56 kDa
      FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector. Review by Michal Bell submitted 19 August 2004

      FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector. Review by Michal Bell submitted 19 August 2004
    • Anti-FOXC2 antibody - ChIP Grade (ab5060) at 1 µg/ml + Human breast cancer lysates at 30 µg
      Developed using the ECL technique

      Predicted band size : 56 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)

      The primary antibody incubated for 1 hour.

    Anti-FOXC2 antibody - ChIP Grade (ab5060)参考文献

    This product has been referenced in:
    • Bornachea O  et al. EMT and induction of miR-21 mediate metastasis development in Trp53-deficient tumours. Sci Rep 2:434 (2012). WB ; Mouse . Read more (PubMed: 22666537) »
    • Kanady JD  et al. Connexin37 and Connexin43 deficiencies in mice disrupt lymphatic valve development and result in lymphatic disorders including lymphedema and chylothorax. Dev Biol 354:253-66 (2011). Mouse . Read more (PubMed: 21515254) »

    See all 11 Publications for this product

    Product Wall

    Thank you for your reply.

    We do not batch test ab5060 in IHC on frozen sections, we only guarantee it to work in IHC-P, ChIP and western blot. Therefore that would explain why you are not getting similar results, as we do not test for this ap...

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    Thank you for contacting Abcam.

    The antibody is covered under our Abpromise for six months and is guaranteed to work in WB, ChIP and IHC-P on mouse and human samples. Since you ordered it in July last year, that is outside the six month guara...

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    I have been able to open the reference Dagenais SL et al. Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome. Gene Expr Patterns 4:611-9 (2004) which has used the antibody in immunohist...

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    I am delighted to hear you are satisfied with the replacement vial sent following your problem reported on the 3rd March; thank you for taking the time to let me know. Please consider submitting a review and in return we will award you 50 points wit...

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    Thank you for your phone call. Originally, this product was released as a fast-track antibody and recently we have upgraded it. One of our customers has tested and used it successfully for application in IHC-formalin-fixed paraffin-embedded sections. I...

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    I'm sorry to hear you are having a problem with your recent vial of ab5060. I can certainly offer you a replacement vial if you can provide me with your order details (the Abcam order number of that particular orde and PO number) and I will arrange...

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    Thank you for your enquiry. All the information available for this product is listed on the on-line datasheet Originally, this product has been released as a fast-track antibody and recently we have upgraded it. One of our customers has tested and ...

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