• 产品名称Anti-FOXA2抗体
    参阅全部 FOXA2 一抗
  • 描述
    兔多克隆抗体to FOXA2
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • 阳性对照
    • Recombinant FOXA2 protein expressed in induced culture of E.coli (one shot Top10 competent cells).



Our Abpromise guarantee covers the use of ab82827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
    • 序列相似性Contains 1 fork-head DNA-binding domain.
    • 翻译后修饰Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
    • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Forkhead box A2 antibody
      • Forkhead box protein A2 antibody
      • FOX A2 antibody
      • foxa2 antibody
      • FOXA2_HUMAN antibody
      • Hepatic nuclear factor 3 beta antibody
      • Hepatocyte nuclear factor 3 antibody
      • Hepatocyte nuclear factor 3 beta antibody
      • Hepatocyte nuclear factor 3-beta antibody
      • HNF 3B antibody
      • HNF-3-beta antibody
      • HNF-3B antibody
      • HNF3B antibody
      • MGC19807 antibody
      • TCF 3B antibody
      • TCF-3B antibody
      • TCF3B antibody
      • Transcription factor 3B antibody
      see all

    Anti-FOXA2 antibody 图像

    • All lanes : Anti-FOXA2 antibody (ab82827) at 1/1000 dilution

      Lane 1 : Uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : Induced culture of E.coli (one shot Top10 competent cells)

      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    Anti-FOXA2 antibody (ab82827)参考文献

    ab82827 has not yet been referenced specifically in any publications.

    Product Wall

    Vielen Dank für Ihren Anruf.

    Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe...

    Read More