概述

  • 产品名称Anti-FOXA2抗体
    参阅全部 FOXA2 一抗
  • 描述
    小鼠单克隆抗体to FOXA2
  • 经测试应用适用于: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment with tag: HLKPEHHYAF NHPFSINNLM SSEQQHHHSH HHHQPHKMDL KAYEQVMHYP GYGSPMPGSL AMGPVTNKTG LDASPLAADT SYYQGVYSRP IMNSS, corresponding to amino acids 363-458 of Human FOXA2

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab60721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
EMSA Use at an assay dependent concentration. PubMed: 20650961
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged FOXA2 is approximately 0.03ng/ml as a capture antibody.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • 序列相似性Contains 1 fork-head DNA-binding domain.
  • 翻译后修饰Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

Anti-FOXA2 antibody 图像

  • Anti-FOXA2 antibody (ab60721) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,42 kDa. We are unsure as to the identity of these extra bands.
  • ab60721 at 10 ug/ml staining FOXA2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HepG2 cells stained with ab60721 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60721, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-FOXA2 antibody (ab60721)参考文献

This product has been referenced in:
  • Yu JS  et al. PI3K/mTORC2 regulates TGF-ß/Activin signalling by modulating Smad2/3 activity via linker phosphorylation. Nat Commun 6:7212 (2015). WB ; Human . Read more (PubMed: 25998442) »
  • Pennarossa G  et al. Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells. Proc Natl Acad Sci U S A 110:8948-53 (2013). Human . Read more (PubMed: 23696663) »

See all 4 Publications for this product

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Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie von Ihnen erwünscht, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben mit dem alternativen Mau...

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Leider waren die Bilder nicht im Anhang. Können Sie diese uns nochmals senden bitte? Vielen Dank.

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Es tut mir leid, dass Sie Probleme mit unserem Antik...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"