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Our Abpromise guarantee covers the use of ab5074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ICC/IF||1/50. PubMed: 19564924|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FOXA2 peptide (ab23065). A nuclear extraction procedure may be required to concentrate the protein in samples.|
Primary incubation was 1 hour. Detected by chemiluminescence.